The composition of the pulmonary microbiota in sarcoidosis - an observational study

被引：19

作者：

Becker, Andre ^{[1
]}

Vella, Giovanna ^{[1
]}

Galata, Valentina ^{[2
]}

Rentz, Katharina ^{[1
]}

Beisswenger, Christoph ^{[1
]}

Herr, Christian ^{[1
]}

Walter, Joern ^{[3
]}

Tierling, Sascha ^{[3
]}

Slevogt, Hortense ^{[4
]}

Keller, Andreas ^{[2
]}

Bals, Robert ^{[1
]}

机构：

[1] Saarland Univ Hosp, Dept Internal Med Pulmonol Allergol Crit Care Med, Homburg, Germany

[2] Saarland Univ, Chair Clin Bioinformat, Saarbrucken, Germany

[3] Univ Saarland, Fac NT, Dept Genet Epigenet, Saarbrucken, Germany

[4] Jena Univ Hosp, Sept Res Ctr, Jena, Germany

来源：

RESPIRATORY RESEARCH | 2019年 / 20卷 / 1期

关键词：

Microbiome; Sarcoidosis; Interstitial lung disease; Inflammation; Infection; MYCOBACTERIAL CATALASE-PEROXIDASE; LYMPH-NODES; RESPONSES; ANTIGENS; TISSUE;

D O I：

10.1186/s12931-019-1013-2

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

BackgroundSarcoidosis is a systemic disease of unknown etiology. The disease mechanisms are largely speculative and may include the role microbial patterns that initiate and drive an underlying immune process. The aim of this study was to characterize the microbiota of the lung of patients with sarcoidosis and compare its composition and diversity with the results from patients with other interstitial lung disease (ILD) and historic healthy controls.MethodsPatients (sarcoidosis, n=31; interstitial lung disease, n=19) were recruited within the PULMOHOM study, a prospective cohort study to characterize inflammatory processes in pulmonary diseases. Bronchoscopy of the middle lobe or the lingula was performed and the recovered fluid was immediately sent for analysis of the pulmonary microbiota by 16sRNA gene sequencing. Subsequent bioinformatic analysis was performed to compare the groups.ResultsThere were no significant differences between patients with sarcoidosis or other ILDs with regard to microbiome composition and diversity. In addition, the abundance of the genera Atopobium, Fusobacterium, Mycobacterium or Propionibacterium were not different between the two groups. There were no gross differences to historical healthy controls.ConclusionThe analysis of the pulmonary microbiota based on 16sRNA gene sequencing did not show a significant dysbiosis in patients with sarcoidosis as compared to other ILD patients. These data do not exclude a microbiological component in the pathogenesis of sarcoidosis.

引用

页数：9

共 20 条

[1] Peripheral blood responses to specific antigens and CD28 in sarcoidosis [J].

Ahmadzai, Hasib ;

Cameron, Barbara ;

Chui, Jeanie J. Y. ;

Lloyd, Andrew ;

Wakefield, Denis ;

Thomas, Paul S. .

RESPIRATORY MEDICINE, 2012, 106 (05) :701-709

[2] The Etiologic Role of Infectious Antigens in Sarcoidosis Pathogenesis [J].

Celada, Lindsay J. ;

Hawkins, Charlene ;

Drake, Wonder P. .

CLINICS IN CHEST MEDICINE, 2015, 36 (04) :561-+

[3] T Cell Responses to Mycobacterial Catalase-Peroxidase Profile a Pathogenic Antigen in Systemic Sarcoidosis [J].

Chen, Edward S. ;

Wahlstrom, Jan ;

Song, Zhimin ;

Willett, Matthew H. ;

Wiken, Maria ;

Yung, Rex C. ;

West, Erin E. ;

McDyer, John F. ;

Zhang, Ying ;

Eklund, Anders ;

Grunewald, Johan ;

Moller, David R. .

JOURNAL OF IMMUNOLOGY, 2008, 181 (12) :8784-8796

[4] Microbial Lineages in Sarcoidosis A Metagenomic Analysis Tailored for Low-Microbial Content Samples [J].

Clarke, Erik L. ;

Lauder, Abigail P. ;

Hofstaedter, Casey E. ;

Hwang, Young ;

Fitzgerald, Ayannah S. ;

Imai, Ize ;

Biernat, Wojciech ;

Rekawiecki, Bartlomiej ;

Majewska, Hanna ;

Dubaniewicz, Anna ;

Litzky, Leslie A. ;

Feldman, Michael D. ;

Bittinger, Kyle ;

Rossman, Milton D. ;

Patterson, Karen C. ;

Bushman, Frederic D. ;

Collman, Ronald G. .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2018, 197 (02) :225-234

[5] Bacterial Topography of the Healthy Human Lower Respiratory Tract [J].

Dickson, Robert P. ;

Erb-Downward, John R. ;

Freeman, Christine M. ;

McCloskey, Lisa ;

Falkowski, Nicole R. ;

Huffnagle, Gary B. ;

Curtis, Jeffrey L. .

MBIO, 2017, 8 (01)

[6] The Microbiome and the Respiratory Tract [J].

Dickson, Robert P. ;

Erb-Downward, John R. ;

Martinez, Fernando J. ;

Huffnagle, Gary B. .

ANNUAL REVIEW OF PHYSIOLOGY, VOL 78, 2016, 78 :481-504

[7] Proteomic Analysis of Kveim Reagent Identifies Targets of Cellular Immunity in Sarcoidosis [J].

Eberhardt, Christian ;

Thillai, Muhunthan ;

Parker, Robert ;

Siddiqui, Nazneen ;

Potiphar, Lee ;

Goldin, Rob ;

Timms, John F. ;

Wells, Athol U. ;

Kon, Onn M. ;

Wickremasinghe, Melissa ;

Mitchell, Donald ;

Weeks, Mark E. ;

Lalvani, Ajit .

PLOS ONE, 2017, 12 (01)

[8] Disordered Toll-like receptor 2 responses in the pathogenesis of pulmonary sarcoidosis [J].

Gabrilovich, M. I. ;

Walrath, J. ;

van Lunteren, J. ;

Nethery, D. ;

Seifu, M. ;

Kern, J. A. ;

Harding, C. V. ;

Tuscano, L. ;

Lee, H. ;

Williams, S. D. ;

Mackay, W. ;

Tomashefski, J. F., Jr. ;

Silver, R. F. .

CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2013, 173 (03) :512-522

[9] Microbial communities in the respiratory tract of patients with interstitial lung disease [J].

Garzoni, Christian ;

Brugger, Silvio D. ;

Qi, Weihong ;

Wasmer, Sarah ;

Cusini, Alexia ;

Dumont, Philippe ;

Gorgievski-Hrisoho, Meri ;

Muehlemann, Kathrin ;

von Garnier, Christophe ;

Hilty, Markus .

THORAX, 2013, 68 (12) :1150-1156

[10] Microbes Are Associated with Host Innate Immune Response in Idiopathic Pulmonary Fibrosis [J].

Huang, Yong ;

Ma, Shwu-Fan ;

Espindola, Milena S. ;

Vij, Rekha ;

Oldham, Justin M. ;

Huffnagle, Gary B. ;

Erb-Downward, John R. ;

Flaherty, Kevin R. ;

Moore, Beth B. ;

White, Eric S. ;

Zhou, Tong ;

Li, Jianrong ;

Lussier, Yves A. ;

Han, MeiLan K. ;

Kaminski, Naftali ;

Garcia, Joe G. N. ;

Hogaboam, Cory M. ;

Martinez, Fernando J. ;

Noth, Imre .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2017, 196 (02) :208-219

← 1 2 →