Colchicine Modulates Oxidative Stress in Serum and Neutrophil of Patients with Behcet Disease Through Regulation of Ca2+ Release and Antioxidant System

被引:37
作者
Korkmaz, Selma [2 ]
Erturan, Ijlal [2 ]
Naziroglu, Mustafa [1 ]
Uguz, Abdulhadi Cihangir [1 ]
Cig, Bilal [1 ]
Ovey, Ishak Suat [1 ]
机构
[1] Suleyman Demirel Univ, Fac Med, Dept Biophys, TR-32260 Isparta, Turkey
[2] Suleyman Demirel Univ, Fac Med, Dept Dermatol, TR-32260 Isparta, Turkey
关键词
Antioxidant; Behcet disease; Calcium ion; Colchicine; Inflammation; Oxidative stress; GLUTATHIONE-PEROXIDASE; SELENIUM; PATHOGENESIS; ACTIVATION; MECHANISMS; ETIOLOGY; PLASMA; CELLS;
D O I
10.1007/s00232-011-9404-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Beh double dagger et disease (BD) is a chronic, inflammatory, and multisystemic condition with an uncertain pathogenesis. One of the major immunologic findings in BD pathogenesis is increase in activity of neutrophil. An increase in the cytosolic free Ca2+ [Ca2+](i) concentration that induces Ca2+ signaling is an important step that participates in the neutrophil activation and reactive oxygen species production that leads to tissue damage in body cells. We aimed to investigate the effects of colchicine on oxidative stress and Ca2+ release in serum and neutrophil of BD patients with active and inactive periods. Twelve Beh double dagger et patients (6 active and 6 inactive) and 6 control subject were included in the study. Disease activity was considered by clinical findings. Serum and neutrophil samples were obtained from the patients and control subjects. Neutrophils from patients with active BD were divided into three subgroups and were incubated with colchicine, verapamil + diltiazem, and colchicine + verapamil + diltiazem, respectively. Erythrocyte sedimentation rate, leucocytes counts, serum C-reactive protein, neutrophil, and serum lipid peroxidation and intracellular Ca2+ release levels were higher in active and inactive groups than in the control group, although their levels were lower in active group than in inactive group. However, neutrophil Ca2+ release levels were decreased in colchicine, verapamil + diltiazem, and colchicine + verapamil + diltiazem groups group compared to active group. Serum glutathione, vitamin A, vitamin E, and beta-carotene concentrations were lower in active and inactive groups than in the control group, although serum vitamin E and beta-carotene concentrations were higher in the inactive group than in the active group. Neutrophil and serum glutathione peroxidase activity within the three groups did not change. In conclusion, we observed the importance of Ca2+ influx into the neutrophils and oxidative stress in the pathogenesis and activation of the patients with BD. Colchicine induced protective effects on oxidative stress by modulating Ca2+ influx in BD patients.
引用
收藏
页码:113 / 120
页数:8
相关论文
共 33 条
  • [1] [Anonymous], 2008, Cell Membranes and Free Radical Research
  • [2] Ca2+ influx shutdown during neutrophil apoptosis:: importance and possible mechanism
    Ayub, K
    Hallett, MB
    [J]. IMMUNOLOGY, 2004, 111 (01) : 8 - 12
  • [3] Involvement of chemokines and Th1 cytokines in the pathogenesis of mucocutaneous lesions of Behqet's disease
    Ben Ahmed, M
    Houman, H
    Miled, M
    Dellagi, K
    Louzir, H
    [J]. ARTHRITIS AND RHEUMATISM, 2004, 50 (07): : 2291 - 2295
  • [4] Plasma membrane localization and function of TRPC1 is dependent on its interaction with β-tubulin in retinal epithelium cells
    Bollimuntha, S
    Cornatzer, E
    Singh, BB
    [J]. VISUAL NEUROSCIENCE, 2005, 22 (02) : 163 - 170
  • [5] Regulation of superoxide production in neutrophils: role of calcium influx
    Brechard, Sabrina
    Tschirhart, Eric J.
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2008, 84 (05) : 1223 - 1237
  • [6] Vascular endothelial function and oxidative stress mechanisms in patients with Behcet's syndrome
    Chambers, JC
    Haskard, DO
    Kooner, JS
    [J]. JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2001, 37 (02) : 517 - 520
  • [7] DESAI ID, 1984, METHOD ENZYMOL, V105, P138, DOI 10.1016/S0076-6879(84)05019-9
  • [8] Behet's disease: infectious aetiology, new autoantigens, and HLA-B51
    Direskeneli, H
    [J]. ANNALS OF THE RHEUMATIC DISEASES, 2001, 60 (11) : 996 - 1002
  • [9] Adenosine deaminase enzyme activity is increased and negatively correlates with catalase, superoxide dismutase and glutathione peroxidase in patients with Behcet's disease:: original contributions/clinical and laboratory investigations
    Erkiliç, K
    Evereklioglu, C
    Çekmen, M
    Özkiris, A
    Duygulu, F
    Dogan, H
    [J]. MEDIATORS OF INFLAMMATION, 2003, 12 (02) : 107 - 116
  • [10] Reduced levels of intracellular calcium releasing in spermatozoa from asthenozoospermic patients
    Espino, Javier
    Mediero, Matias
    Lozano, Graciela M.
    Bejarano, Ignacio
    Ortiz, Agueda
    Garcia, Juan F.
    Pariente, Jose A.
    Rodriguez, Ana B.
    [J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2009, 7