Development of a Polymerase Chain Reaction Lateral Flow Immunoassay for Rapid Authentication of Venison in Food Products

被引：5

作者：

Zhao, Liangjuan ^{[1
]}

Yu, Zhilong ^{[2
,3
]}

Liu, Jinyu ^{[1
]}

Zhang, Hongwei ^{[4
]}

Hu, Yaxi ^{[2
]}

Lu, Xiaonan ^{[2
,3
]}

Zheng, Wenjie ^{[1
]}

机构：

[1] Tianjin Normal Univ, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China

[2] Univ British Columbia, Fac Land & Food Syst, Food Nutr & Hlth Program, Vancouver, BC V6T 1Z4, Canada

[3] McGill Univ, Fac Agr & Environm Sci, Dept Food Sci & Agr Chem, Ste Anne De Bellevue, PQ H9X 3V9, Canada

[4] Anim & Plant & Foodstuffs Inspect Ctr Tianjin Cus, Tianjin 300387, Peoples R China

来源：

ACS FOOD SCIENCE & TECHNOLOGY | 2021年 / 1卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

venison product; meat authenticity; polymerase chain reaction; lateral flow strip; biosensor; MEAT; IDENTIFICATION; PCR; ADULTERATION; ASSAY; BEEF;

D O I：

10.1021/acsfoodscitech.0c00085

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A novel polymerase chain reaction lateral flow immunoassay (PCR-LFI) test was developed to detect venison in food products in a rapid, inexpensive, and user-friendly manner. The LFI strips allowed the detection of PCR products within 5 min by reading the color signals with the naked eye. The PCR-LFI test uncovered a high specificity for venison with no cross-reactivity to 19 animal and plant species and enabled the detection of raw, oven-heated, and fried venison in binary mixtures with a limit of detection (LOD) of 0.01% (w/w), which was lower than the LOD of PCR agarose gel electrophoresis [0.1% (w/w)]. In addition, the PCR-LFI test was applied to detect 15 commercial venison products, and the results were validated by PCR agarose gel electrophoresis. Given its superiority in terms of cost, reliability, and simplicity, the PCR-LFI test has great potential to be employed as a meat authentication tool in the food industry.

引用

页码：12 / 16

页数：5

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