Retinal capillary oximetry with visible light optical coherence tomography

被引：43

作者：

Pi, Shaohua ^{[1
]}

Hormel, Tristan T. ^{[1
]}

Wei, Xiang ^{[1
]}

Cepurna, William ^{[1
]}

Wang, Bingjie ^{[1
]}

Morrison, John C. ^{[1
]}

Jia, Yali ^{[1
]}

机构：

[1] Oregon Hlth & Sci Univ, Casey Eye Inst, Portland, OR 97239 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2020年 / 117卷 / 21期

基金：

美国国家卫生研究院;

关键词：

capillary oximetry; visible light OCT; retinal capillary; blood oxygenation; vascular biology and physiology; OXYGEN-CONSUMPTION; HIGH-RESOLUTION; ANGIOGRAPHY; RETINOPATHY; SATURATION; VESSELS; CELLS;

D O I：

10.1073/pnas.1918546117

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Assessing oxygen saturation (sO(2)) remains challenging but is nonetheless necessary for understanding retinal metabolism. We and others previously achieved oximetry on major retinal vessels and measured the total retinal oxygen metabolic rate in rats using visible-light optical coherence tomography. Here we extend oximetry measurements to capillaries and investigate all three retinal vascular plexuses by amplifying and extracting the spectroscopic signal from each capillary segment under the guidance of optical coherence tomography (OCT) angiography. Using this approach, we measured capillary sO(2) in the retinal circulation in rats, demonstrated reproducibility of the results, validated the measurements in superficial capillaries with known perfusion pathways, and determined sO(2) responses to hypoxia and hyperoxia in the different retinal capillary beds. OCT capillary oximetry has the potential to provide new insights into the retinal circulation in the normal eye as well as in retinal vascular diseases.

引用

页码：11658 / 11666

页数：9

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