Overexpression of Zm-HINT1 in Arabidopsis thaliana enhances resistance to Fusarium graminearum

被引：4
作者：
Wu, Liuji [1 ,2 ,3 ]
Zhang, Huimin [1 ,2 ,3 ]
Zhang, Jun [1 ,2 ,3 ]
Wu, Liancheng [1 ,2 ,3 ]
Xi, Zhangying [1 ,2 ,3 ]
Chen, Yanhui [1 ,2 ,3 ]
机构：
[1] Henan Agr Univ, Zhengzhou 450002, Peoples R China
[2] Synerget Innovat Ctr Henan Grain Crops, Zhengzhou 450002, Peoples R China
[3] Key Lab Physiol Ecol & Genet Improvement Food Cro, Zhengzhou 450002, Peoples R China
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2015年 / 121卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Histidine triad nucleotide binding protein 1(HINT1); Maize; Subcellular localization; Real-time PCR; Defense response; Crop breeding; AGROBACTERIUM-MEDIATED TRANSFORMATION; NUCLEOTIDE-BINDING PROTEIN; TUMOR-SUPPRESSOR; EXPRESSION; HINT1; PKCI/HINT1; GROWTH; GENE;
D O I：
10.1007/s11240-015-0709-3
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Histidine triad nucleotide binding protein 1(HINT1) plays an important role in many biological processes especially in cell biology, and they have been found in a wide variety of species. However, the functional attributes of HINT1 homologues in plants have not yet been reported. We have previously isolated the full-length cDNA of an HINT1 homologue from maize (Zea mays L.), designated as Zm-HINT1. Subcellular localization was analysed after particle-mediated transient transformation of onion epidermal cells. The result revealed that Zm-HINT1 was targeted to the nucleus. Based on real-time PCR analysis, the expression level of Zm-HINT1 increased significantly after salicylic acid or jasmonic acid treatments. To further investigate the potential physiological role of Zm-HINT1, a binary vector was constructed for Zm-HINT1 synthesis and transgenic Arabidopsis lines were generated by Agrobacterium-mediated transformation. Transgenic Zm-HINT1 plants showed increased resistance to Fusarium graminearum compared to the wild type and control plants. Futhermore, real-time PCR results revealed that the pathogen defense genes pathogenesis-related 4 (PR-4) and a peroxidase were upregulated by Zm-HINT1 overexpression. Thus, our data demonstrated that Zm-HINT1 induced antifungal activity against Fusarium graminearum and it may be useful for crop breeding in improving biotic stress tolerance.
引用
收藏
页码：511 / 518
页数：8
相关论文
共 37 条
[1]   The neurodegenerative disease protein aprataxin resolves abortive DNA ligation intermediates [J].
Ahel, Ivan ;
Rass, Ulrich ;
El-Khamisy, Sherif F. ;
Katyal, Sachin ;
Clements, Paula M. ;
McKinnon, Peter J. ;
Caldecott, Keith W. ;
West, Stephen C. .
NATURE, 2006, 443 (7112) :713-716
[2]   The defense response in Arabidopsis thaliana against Fusarium sporotrichioides [J].
Asano, Tomoya ;
Kimura, Makoto ;
Nishiuchi, Takumi .
PROTEOME SCIENCE, 2012, 10
[3]   Molecular cloning of SoHPPR encoding a hydroxyphenylpyruvate reductase, and its expression in cell suspension cultures of Salvia officinalis [J].
Barberini, S. ;
Savona, M. ;
Raffi, D. ;
Leonardi, M. ;
Pistelli, La. ;
Stochmal, A. ;
Vainstein, A. ;
Pistelli, Lu. ;
Ruffoni, B. .
PLANT CELL TISSUE AND ORGAN CULTURE, 2013, 114 (01) :131-138
[4]   Supersensitivity to amphetamine in protein kinase-C interacting protein/HINT1 knockout mice [J].
Barbier, Elisabeth ;
Zapata, Agustin ;
Oh, Eric ;
Liu, Qing ;
Zhu, Fei ;
Undie, Ashiwel ;
Shippenberg, Toni ;
Wang, Jia Bei .
NEUROPSYCHOPHARMACOLOGY, 2007, 32 (08) :1774-1782
[5]   Anti-depressant and anxiolytic like behaviors in PKCI/HINT1 knockout mice associated with elevated plasma corticosterone level [J].
Barbier, Elisabeth ;
Wang, Jia Bei .
BMC NEUROSCIENCE, 2009, 10
[6]  
Bechtold N, 1998, METH MOL B, V82, P259
[7]   Mycotoxins [J].
Bennett, JW ;
Klich, M .
CLINICAL MICROBIOLOGY REVIEWS, 2003, 16 (03) :497-+
[8]   Crystal structures of HINT demonstrate that histidine triad proteins are GalT-related nucleotide-binding proteins [J].
Brenner, C ;
Garrison, P ;
Gilmour, J ;
Peisach, D ;
Ringe, D ;
Petsko, GA ;
Lowenstein, JM .
NATURE STRUCTURAL BIOLOGY, 1997, 4 (03) :231-238
[9]   Reprogramming a maize plant: transcriptional and metabolic changes induced by the fungal biotroph Ustilago maydis [J].
Doehlemann, Gunther ;
Wahl, Ramon ;
Horst, Robin J. ;
Voll, Lars M. ;
Usadel, Bjoern ;
Poree, Fabien ;
Stitt, Mark ;
Pons-Kuehnemann, Joern ;
Sonnewald, Uwe ;
Kahmann, Regine ;
Kaemper, Joerg .
PLANT JOURNAL, 2008, 56 (02) :181-195
[10]   Signal signature of aboveground-induced resistance upon belowground herbivory in maize [J].
Erb, Matthias ;
Flors, Victor ;
Karlen, Danielle ;
de Lange, Elvira ;
Planchamp, Chantal ;
D'Alessandro, Marco ;
Turlings, Ted C. J. ;
Ton, Jurriaan .
PLANT JOURNAL, 2009, 59 (02) :292-302
1234