Complex effects of ryanodine on the sarcoplasmic reticulum Ca2+ levels in smooth muscle cells

被引:13
作者
Gómez-Viquez, L
Rueda, A
García, U
Guerrero-Hernández, A
机构
[1] Inst Politecn Nacl, CINVESTAV, Dept Bioquim, Mexico City 07000, DF, Mexico
[2] Inst Politecn Nacl, CINVESTAV, Dept Fisiol Biofis & Neurociencias, Mexico City 07000, DF, Mexico
关键词
ryanodine receptor; sarcoplasmic reticulutn (SR) Ca2+ level; Mag-Fura-2; caffeine; thapsigargin; SERCA pump; smooth muscle cells;
D O I
10.1016/j.ceca.2005.06.002
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have studied the effects of ryanodine and inhibition of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) with thapsigargin, on both [Ca2+](i) and the sarcoplasmic reticulum (SR) Ca2+ level during caffeine-induced Ca2+ release in single smooth muscle cells. Incubation with 10 mu M ryanodine did not inhibit the first caffeine-induced [Ca2+]; response, although it abolished the [Ca2+]i response to a second application of caffeine. To assess whether ryanodine was inducing a permanent depletion of the internal Ca2+ stores, we measured the SR Ca2+ level with Mag-Fura-2. The magnitude of the caffeine-induced reduction in the SR Ca2+ level was not augmented by incubating cells with 1 mu M ryanodine. Moreover, on removal of caffeine, the SR Ca2+ levels partially recovered in 61% of the cells due to the activity of thapsigargin-sensitive SERCA pumps. Unexpectedly, 10 mu M ryanodine instead of inducing complete depletion of SR Ca2+ stores markedly reduced the caffeine-induced SR Ca2+ response. It was necessary to previously inhibit SERCA pumps with thapsigargin for ryanodine to be able to induce caffeine-triggered permanent depletion of SR Ca2+ stores. These data suggest that the effect of ryanodine on smooth muscle SR Ca2+ stores was markedly affected by the activity of SERCA pumps. Our data highlight the importance of directly measuring SR Ca2+ levels to determine the effect of ryanodine on the internal Ca2+ stores. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:121 / 130
页数:10
相关论文
共 46 条
[21]   Ryanodine sensitizes the Ca2+ release channel (ryanodine receptor) to Ca2+ activation [J].
Masumiya, H ;
Li, P ;
Zhang, L ;
Chen, SRW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (43) :39727-39735
[22]  
MEISSNER G, 1986, J BIOL CHEM, V261, P6300
[23]   Ca2+ sparks and Ca2+ waves activate different Ca2+-dependent ion channels in single myocytes from rat portal vein [J].
Mironneau, J ;
Arnaudeau, S ;
MacrezLepretre, N ;
Boittin, FX .
CELL CALCIUM, 1996, 20 (02) :153-160
[24]   In situ characterization of the Ca2+ sensitivity of barge conductance Ca2+-activated K+ channels:: Implications for their use as near-membrane Ca2+ indicators in smooth muscle cells [J].
Muñoz, A ;
García, L ;
Guerrero-Hernández, A .
BIOPHYSICAL JOURNAL, 1998, 75 (04) :1774-1782
[25]   The endoplasmic reticulum as one continuous Ca2+ pool:: visualization of rapid Ca2+ movements and equilibration [J].
Park, MK ;
Petersen, OH ;
Tepikin, AV .
EMBO JOURNAL, 2000, 19 (21) :5729-5739
[26]  
PESSAH IN, 1986, J BIOL CHEM, V261, P8643
[27]  
PESSAH IN, 1987, MOL PHARMACOL, V31, P232
[28]  
POENIE M, 1990, CELL CALCIUM, V2, P85
[29]  
ROUSSEAU E, 1987, AM J PHYSIOL, V253, P364
[30]   The initial inositol 1,4,5-trisphosphate response induced by histamine is strongly amplified by Ca2+ release from internal stores in smooth muscle [J].
Rueda, A ;
García, L ;
Soria-Jasso, LE ;
Arias-Montaño, JA ;
Guerrero-Hernández, A .
CELL CALCIUM, 2002, 31 (04) :161-173