Restricted epigenetic inheritance of H3K9 methylation

被引：204

作者：

Audergon, Pauline N. C. B.

Catania, Sandra

Kagansky, Alexander

Tong, Pin

Shukla, Manu

Pidoux, Alison L.

Allshire, Robin C. ^{[1
]}

机构：

[1] Univ Edinburgh, Max Born Crescent, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3BF, Midlothian, Scotland

来源：

SCIENCE | 2015年 / 348卷 / 6230期

基金：

英国惠康基金;

关键词：

JMJC DOMAIN PROTEIN; FISSION YEAST; HETEROCHROMATIN NUCLEATION; HISTONE DEMETHYLATION; RNA INTERFERENCE; LYSINE; 9; MAINTENANCE; TRANSCRIPTION; ROLES; REPLICATION;

D O I：

10.1126/science.1260638

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Posttranslational histone modifications are believed to allow the epigenetic transmission of distinct chromatin states, independently of associated DNA sequences. Histone H3 lysine 9 (H3K9) methylation is essential for heterochromatin formation; however, a demonstration of its epigenetic heritability is lacking. Fission yeast has a single H3K9 methyltransferase, Clr4, that directs all H3K9 methylation and heterochromatin. Using releasable tethered Clr4 reveals that an active process rapidly erases H3K9 methylation from tethering sites in wild-type cells. However, inactivation of the putative histone demethylase Epe1 allows H3K9 methylation and silent chromatin maintenance at the tethering site through many mitotic divisions, and transgenerationally throughmeiosis, after release of tethered Clr4. Thus, H3K9 methylation is a heritable epigenetic mark whose transmission is usually countered by its active removal, which prevents the unauthorized inheritance of heterochromatin.

引用

页码：132 / 135

页数：4

共 30 条

[1] Functional mammalian homologues of the Drosophila PEV-modifier Su(var)3-9 encode centromere-associated proteins which complex with the heterochromatin component M31 [J].

Aagaard, L ;

Laible, G ;

Selenko, P ;

Schmid, M ;

Dorn, R ;

Schotta, G ;

Kuhfittig, S ;

Wolf, A ;

Lebersorger, A ;

Singh, PB ;

Reuter, G ;

Jenuwein, T .

EMBO JOURNAL, 1999, 18 (07) :1923-1938

[2] Heterochromatin maintenance and establishment Lessons from the mouse pericentromere [J].

Almouzni, Genevieve ;

Probst, Aline V. .

NUCLEUS, 2011, 2 (05) :332-338

[3] A novel jmjC domain protein modulates heterochromatization in fission yeast [J].

Ayoub, N ;

Noma, K ;

Isaac, S ;

Kahan, T ;

Grewal, SIS ;

Cohen, A .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (12) :4356-4370

[4] PKA-dependent regulation of the histone lysine demethylase complex PHF2-ARID5B [J].

Baba, Atsushi ;

Ohtake, Fumiaki ;

Okuno, Yosuke ;

Yokota, Kenichi ;

Okada, Maiko ;

Imai, Yuuki ;

Ni, Min ;

Meyer, Clifford A. ;

Igarashi, Katsuhide ;

Kanno, Jun ;

Brown, Myles ;

Kato, Shigeaki .

NATURE CELL BIOLOGY, 2011, 13 (06) :668-U101

[5] Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain [J].

Bannister, AJ ;

Zegerman, P ;

Partridge, JF ;

Miska, EA ;

Thomas, JO ;

Allshire, RC ;

Kouzarides, T .

NATURE, 2001, 410 (6824) :120-124

[6] The Cul4-Ddb1Cdt2 Ubiquitin Ligase Inhibits Invasion of a Boundary-Associated Antisilencing Factor into Heterochromatin [J].

Braun, Sigurd ;

Garcia, Jennifer F. ;

Rowley, Margot ;

Rougemaille, Mathieu ;

Shankar, Smita ;

Madhani, Hiten D. .

CELL, 2011, 144 (01) :41-54

[7] Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance [J].

Buscaino, Alessia ;

Lejeune, Erwan ;

Audergon, Pauline ;

Hamilton, Georgina ;

Pidoux, Alison ;

Allshire, Robin C. .

EMBO JOURNAL, 2013, 32 (09) :1250-1264

[8] RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond [J].

Castel, Stephane E. ;

Martienssen, Robert A. .

NATURE REVIEWS GENETICS, 2013, 14 (02) :100-112

[9] Cell cycle control of centromeric repeat transcription and heterochromatin assembly [J].

Chen, Ee Sin ;

Zhang, Ke ;

Nicolas, Estelle ;

Cam, Hugh P. ;

Zofall, Martin ;

Grewal, Shiv I. S. .

NATURE, 2008, 451 (7179) :734-737

[10] Structural and Functional Coordination of DNA and Histone Methylation [J].

Cheng, Xiaodong .

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2014, 6 (08)

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