Amino-terminal fragment of urokinase-type plasminogen activator inhibits HIV-1 replication

被引:26
作者
Wada, M
Wada, NA
Shirono, H
Taniguchi, K
Tsuchie, H
Koga, J
机构
[1] JCR Pharmaceut Co Ltd, Lab Bioengn & Res, Nishi Ku, Kobe, Hyogo 6512241, Japan
[2] Eiken Chem Co Ltd, Nasu Labs, Ohtahara, Tochigi 3240036, Japan
[3] Osaka Univ, Microbial Dis Res Inst, Suita, Osaka 5650871, Japan
关键词
HIV; AIDS; CD8; uPA; ATF; uPAR; lipid raft; assembly; budding; GPI-anchored protein;
D O I
10.1006/bbrc.2001.4965
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CD8(+) T lymphocytes have been shown to produce unidentified soluble factors active in suppressing HIV-1 replication. In this study, we purified an HIV-1 suppressing activity from the culture supernatant of an immortalized CD8(+) T cell clone, derived from an HIV-1 infected long-term nonprogressor, and identified this activity as the amino-terminal fragment (ATF) of urokinase-type plasminogen activator (uPA), ATF is catalytically inactive, but suppresses the release of viral particles from the HIV-1 infected cell lines via binding to its receptor CD87, In contrast, cell proliferation and the secretion of an HIV-1 LTR driven reporter gene product were not affected by ATF. These findings suggest that ATF may inhibit the assembly and budding of HIV-1, which provides a novel therapeutic strategy for AIDS. (C) 2001 Academic Press.
引用
收藏
页码:346 / 351
页数:6
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