Dissecting promoter of InMYB1 gene showing petal-specific expression

被引:3
|
作者
Azuma, Mirai [1 ,2 ]
Oshima, Yoshimi [3 ]
Sakamoto, Shingo [3 ]
Mitsuda, Nobutaka [3 ]
Ohme-Takagi, Masaru [3 ,4 ]
Otagaki, Shungo [1 ]
Matsumoto, Shogo [1 ]
Shiratake, Katsuhiro [1 ]
机构
[1] Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[2] Nihon Univ, Dept Agr Biosci, Coll Bioresource Sci, Fujisawa, Kanagawa 2520880, Japan
[3] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Higashi Ku, Tsukuba, Ibaraki 3058566, Japan
[4] Saitama Univ, Inst Environm Sci & Technol, Sakura Ku, Saitama 3388570, Japan
基金
日本科学技术振兴机构; 日本学术振兴会;
关键词
cis-element; InMYB1; promoter; petal-specific gene expression; TRANSCRIPTION; FLOWERS; IDENTIFICATION; TRANSFORMATION; ELEMENTS; DATABASE; PATHWAY; REGION; BLUE;
D O I
10.5511/plantbiotechnology.18.0529a
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We had previously reported that the InMYB1 promoter, the 1023 bp upstream region of InMYB1, works petalspecifically in various dicot plants by recognizing petal identity at a cellular level. To determine the petal-specific region in the InMYB1 promoter, Arabidopsis plants harboring InMYB1_1023b:: GUS (beta-glucuronidase), InMYB1_713b::GUS, InMYB1_506b::GUS, InMYB1_403b::GUS, InMYB1_332b::GUS, InMYB1_200b::GUS and InMYB1_140b:: GUS were produced and confirmed a shortest region, which has the petal-specific promoter activity by using histochemical GUS assay. Petal-specific GUS staining was not observed in the Arabidopsis plants transformed with InMYB1_200b:: GUS and InMYB1_140b:: GUS, but observed in transgenic Arabidopsis plants harboring from InMYB1_1023b:: GUS to InMYB1_332b:: GUS. cDNA sequence of InMYB1 shows that 120 bp upstream region of InMYB1 is 5' untranslated region, suggesting that the 332-121 bp upstream region of InMYB1 contains an important element for petal-specific gene expression. In the Arabidopsis harboring the InMYB1_332-121bx3_TATA_O::GUS, petal-specific GUS staining was observed and the staining was stronger than in the Arabidopsis harboring InMYB1_1023b::GUS. This result shows that the 332-121 bp region is enough and essential for the petal specificity and the InMYB1_332-121bx3_TATA_O could be used for the molecular breeding of floricultural crops.
引用
收藏
页码:243 / 248
页数:6
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