Partitioning of Ciprofloxacin in aqueous two-phase system of poly(ethylene glycol) and sodium sulphate

被引：85

作者：

Mokhtarani, Babak ^{[2
]}

Karimzadeh, Ramin ^{[1
]}

Amini, Mohammad Hassan ^{[2
]}

Manesh, Siavash Darvish ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Dept Chem Engn, Tehran, Iran

[2] Chem & Chem Engn Res Ctr Iran, Tehran, Iran

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2008年 / 38卷 / 02期

关键词：

aqueous two-phase system; ATPS; partitioning; Ciprofloxacin; experimental design;

D O I：

10.1016/j.bej.2007.07.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Extraction by an aqueous two-phase system is a powerful technique for separation, concentration, and purification of biomolecules and pharmaceutical products. In the present study, the partitioning of Ciprofloxacin in aqueous two-phase system of PEG-Na2SO4-water has been investigated. The influences of temperature, salt concentration, polymer concentration and polymer molecular weight on the partitioning of Ciprofloxacin were studied. The experiments were designed using an orthogonal central composite design (orthogonal CCD) based on 2(3) full factorial and a second-order model was used to determine the effects of different factors on Ciprofloxacin partitioning. The results of the model indicate that the Ciprofloxacin partitioning is highly dependent on salt concentration. Temperature and PEG concentration have moderate effects on partitioning but PEG molecular weight has no significant effect on the antibiotic partitioning. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：241 / 247

页数：7

共 14 条

[1]

Albertsson P.A., 1986, PARTITION CELL PARTI

[2]

Beijerinck M. W., 1896, ZBL BAKT, V2, P698

[3] Experimental results and thermodynamic modeling of the partitioning of lysozyme, bovine serum albumin, and α-amylase in aqueous two-phase systems of PEG and (K2HPO4 or Na2SO4) [J].