Resolution in three-photon fluorescence scanning microscopy

被引：55

作者：

Gu, M

机构：

[1] Department of Applied Physics, Victoria University of Technology, Vic. 8001

来源：

OPTICS LETTERS | 1996年 / 21卷 / 13期

关键词：

D O I：

10.1364/OL.21.000988

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Resolution in potential three-photon fluorescence scanning microscopy is discussed in terms of the three-dimensional optical transfer function. Images of layers and sharp edges are presented for a comparison of the resolution with that in two-photon fluorescence microscopic imaging. For the same fluorescence wavelength the resolution is almost the same in both cases. However, for a given illumination wavelength the resolution for imaging a thick object in the case of three-photon fluorescence imaging can be improved by as much as 40-50% relative to that in two-photon imaging. (C) 1996 Optical Society of America

引用

页码：988 / 990

页数：3

共 8 条

[1]

[Anonymous], 1995, COMMUNICATION

[2] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].