HSV induces an early primary Th1 CD4 T cell response in neonatal mice, but reduced CTL activity at the time of the peak adult response

被引:15
作者
Evans, IAC
Jones, CA
机构
[1] Childrens Hosp, Herpesvirus Res Unit, Westmead, NSW 2145, Australia
[2] Univ Sydney, Discipline Pediat & Child Hlth, Sydney, NSW 2006, Australia
[3] Childrens Hosp, Dept Immunol & Infect Dis, Sydney, NSW, Australia
关键词
neonate; Th1/Th2; cells; CTL; herpes simplex virus;
D O I
10.1002/eji.200425333
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Neonates are highly susceptible to HSV. In this study, we analyzed the primary neonatal cell-mediated response to HSV at the site of T cell activation, the draining lymph nodes (LN), and examined the effects of dose and the ability of HSV to replicate on the strength and character of this response. Neonatal mice mounted a predominantly Th1 cytokine (IFN-gamma) response at all doses of a replication-competent thymidine kinase-negative HSV-2 strain (186 Delta Kpn) and at high doses of a replication-defective HSV-2 virus (dl5-29, UL5(-)/UL29(-)). Both neonates and adults showed increased production of Th2 cytokines (IL-4 and/or IL-5) at high doses of the replication-defective or inactivated HSV strains. An age-dependent difference in the strength of the Th1 response was noted, with neonates mounting adult-like responses at low but not high doses of HSV. Neonatal mice also showed impaired CD8(+) T cell activation and reduced HSV-specific CTL effector function at the time of the peak adult response. These studies are the first to highlight the impaired primary neonatal T cell response to HSV in the LN.
引用
收藏
页码:1454 / 1462
页数:9
相关论文
共 38 条
[1]   Neonatal adaptive immunity comes of age [J].
Adkins, B ;
Leclerc, C ;
Marshall-Clarke, S .
NATURE REVIEWS IMMUNOLOGY, 2004, 4 (07) :553-564
[2]  
Adkins B, 1998, J IMMUNOL, V160, P4217
[3]   Exclusive Th2 primary effector function in spleens but mixed Th1/Th2 function in lymph nodes of murine neonates [J].
Adkins, B ;
Bu, YR ;
Cepero, E ;
Perez, R .
JOURNAL OF IMMUNOLOGY, 2000, 164 (05) :2347-2353
[4]   HERPES-SIMPLEX VIRUS TYPE-1-SPECIFIC CYTOTOXIC LYMPHOCYTES-T RECOGNIZE IMMEDIATE-EARLY PROTEIN ICP27 [J].
BANKS, TA ;
ALLEN, EM ;
DASGUPTA, S ;
SANDRIGOLDIN, R ;
ROUSE, BT .
JOURNAL OF VIROLOGY, 1991, 65 (06) :3185-3191
[5]  
Bot A, 2000, Int Rev Immunol, V19, P221, DOI 10.3109/08830180009088506
[6]   Immunization with a replication-deficient mutant of herpes simplex virus type 1 (HSV-1) induces a CD8(+) cytotoxic T-lymphocyte response and confers a level of protection comparable to that of wild-type HSV-1 [J].
Brehm, MA ;
Bonneau, RH ;
Knipe, DM ;
Tevethia, SS .
JOURNAL OF VIROLOGY, 1997, 71 (05) :3534-3544
[7]   DIMINISHED INTERFERON-GAMMA AND LYMPHOCYTE-PROLIFERATION IN NEONATAL AND POSTPARTUM PRIMARY HERPES-SIMPLEX VIRUS-INFECTION [J].
BURCHETT, SK ;
COREY, L ;
MOHAN, KM ;
WESTALL, J ;
ASHLEY, R ;
WILSON, CB .
JOURNAL OF INFECTIOUS DISEASES, 1992, 165 (05) :813-818
[8]   Prevention of the responses is critical for tolerance [J].
Chen, NX ;
Gao, QL ;
Field, EH .
TRANSPLANTATION, 1996, 61 (07) :1076-1083
[9]   Progression of armed CTL from draining lymph node to spleen shortly after localized infection with herpes simplex virus 1 [J].
Coles, RM ;
Mueller, SN ;
Heath, WR ;
Carbone, FR ;
Brooks, AG .
JOURNAL OF IMMUNOLOGY, 2002, 168 (02) :834-838
[10]   Antigen-specific CD8(+) T cell subset distribution in lymph nodes draining the site of herpes simplex virus infection [J].
Cose, SC ;
Jones, CM ;
Wallace, ME ;
Heath, WR ;
Carbone, FR .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (09) :2310-2316