Single-molecule Measurements of DNA Topology and Topoisomerases

被引：24

作者：

Neuman, Keir C. ^{[1
]}

机构：

[1] NHLBI, Lab Mol Biophys, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2010年 / 285卷 / 25期

基金：

美国国家卫生研究院;

关键词：

SUPERCOILED DNA; RNA-POLYMERASE; TRANSLOCATION; RELAXATION; ELASTICITY; MECHANISM; FORCE; IV;

D O I：

10.1074/jbc.R109.092437

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Topological properties of DNA influence its mechanical and biochemical interactions. Genomic DNA is maintained in a state of topological homeostasis by topoisomerases and is subjected to mechanical stress arising from replication and segregation. Despite their fundamental roles, the effects of topology and force have been difficult to ascertain. Developments in single-molecule manipulation techniques have enabled precise control and measurement of the topology of individual DNA molecules under tension. This minireview provides an overview of these single-molecule techniques and illustrates their unique capabilities through a number of specific examples of single-molecule measurements of DNA topology and topoisomerase activity.

引用

页码：18967 / 18971

页数：5

共 44 条

[1] Stretched and overwound DNA forms a Pauling-like structure with exposed bases [J].

Allemand, JF ;

Bensimon, D ;

Lavery, R ;

Croquette, V .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (24) :14152-14157

[2] Structural plasticity of single chromatin fibers revealed by torsional manipulation [J].

Bancaud, A ;

Silva, NCE ;

Barbi, M ;

Wagner, G ;

Allemand, JF ;

Mozziconacci, J ;

Lavelle, C ;

Croquette, V ;

Victor, JM ;

Prunell, A ;

Viovy, JL .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (05) :444-450

[3]

Bates A., 2005, DNA topology

[4] SIGN INVERSION MECHANISM FOR ENZYMATIC SUPERCOILING OF DNA [J].

BROWN, PO ;

COZZARELLI, NR .

SCIENCE, 1979, 206 (4422) :1081-1083

[5] Structural transitions and elasticity from torque measurements on DNA [J].

Bryant, Z ;

Stone, MD ;

Gore, J ;

Smith, SB ;

Cozzarelli, NR ;

Bustamante, C .

NATURE, 2003, 424 (6946) :338-341

[6] DNA topoisomerases: Structure, function, and mechanism [J].

Champoux, JJ .

ANNUAL REVIEW OF BIOCHEMISTRY, 2001, 70 :369-413

[7] Braiding DNA: Experiments, simulations, and models [J].

Charvin, G ;

Vologodskii, A ;

Bensimon, D ;

Croquette, V .

BIOPHYSICAL JOURNAL, 2005, 88 (06) :4124-4136

[8] Tracking topoisomerase activity at the single-molecule level [J].

Charvin, G ;

Strick, TR ;

Bensimon, D ;

Croquette, V .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 2005, 34 :201-219

[9] Twisting DNA: single molecule studies [J].

Charvin, G ;

Allemand, JF ;

Strick, TR ;

Bensimon, D ;

Croquette, V .

CONTEMPORARY PHYSICS, 2004, 45 (05) :383-403

[10] Single-molecule study of DNA unlinking by eukaryotic and prokaryotic type-II topoisomerases [J].

Charvin, G ;

Bensimon, D ;

Croquette, V .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (17) :9820-9825

← 1 2 3 4 5 →