A label-free immunosensor for detecting common acute lymphoblastic leukemia antigen (CD10) based on gold nanoparticles by quartz crystal microbalance

被引：28

作者：

Yan, Zhiyong ^{[1
]}

Yang, Min ^{[1
]}

Wang, Zonghua ^{[1
]}

Zhang, Feifei ^{[1
]}

Xia, Jianfei ^{[1
]}

Shi, Guoyu ^{[1
]}

Xia, Lin ^{[1
]}

Li, Yanhui ^{[1
]}

Xia, Yanzhi ^{[1
]}

Xia, Linhua ^{[1
]}

机构：

[1] Qingdao Univ, Shandong Sinojapanese Ctr Collaborat Res Carbon N, Collaborat Innovat Ctr Marine Biomass Fiber Mat &, Coll Chem Sci & Engn,Lab Fiber Mat & Modern Text, Qingdao 266071, Shandong, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 210卷

基金：

中国国家自然科学基金;

关键词：

CD10; QCM; Label-free; Immunosensor; Au NPs; PROTEIN-KINASE ACTIVITY; PIEZOELECTRIC IMMUNOSENSOR; SIGNAL AMPLIFICATION; QCM; BIOSENSOR; ANTIBODY; IMMUNOASSAY; RECOGNITION; GRAPHENE;

D O I：

10.1016/j.snb.2014.12.104

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Rapid and sensitive detection of the common acute lymphoblastic leukemia antigen (CD10) is crucial for diagnosing and prognosing hematopoietic tumors and several carcinomas. In the present study, a label-free immunosensor for detecting CD10 by Quartz Crystal Microbalance (QCM) was developed. The detection system has a good selectivity based on the biospecific interactions between the CD10 antigen and antibody, and the sensitivity of the immunosensor was further improved for Au NPs not only carried more antibodies but also acted as mass enhancers. Experimental results confirmed that this developed method could be conveniently used for the detection of CD10 without labeling, and CD10 could be measured quantitatively in the concentration range from 1.0 x 10(-8) to 1.0 x 10(-11) M. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：248 / 253

页数：6

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