Concise Review: In Vitro Formation of Bone-Like Nodules Sheds Light on the Application of Stem Cells for Bone Regeneration

被引：37

作者：

Aiami, Saad Mechiche ^{[1
,2
]}

Gangloff, Sophie C. ^{[1
,3
]}

Laurent-Maquin, Dominique ^{[1
,2
,4
]}

Wang, Yun ^{[5
]}

Kerdjoudj, Halima ^{[1
,2
]}

机构：

[1] Univ Reims, EA 4691, SFR CAP Sante, Biomat & Inflammat Site Osseux,Pole Sante,Federat, Reims, France

[2] UFR Odontol, Reims, France

[3] UFR Pharm, Reims, France

[4] Ctr Hosp Univ Reims, Hop Maison Blanche, Pole Odontol, Reims, France

[5] Med Univ South Carolina, Charleston, SC USA

来源：

STEM CELLS TRANSLATIONAL MEDICINE | 2016年 / 5卷 / 11期

关键词：

Bone-like nodule; Osteoblast; Differentiation; Stem cells; Extracellular matrix mineralization; FETAL-RAT CALVARIA; INTRACELLULAR CALCIUM-PHOSPHATE; OSTEOBLAST DIFFERENTIATION; FORMED INVITRO; ENGINEERED BONE; GENE-EXPRESSION; STROMAL CELLS; MINERALIZATION; TISSUE; GROWTH;

D O I：

10.5966/sctm.2015-0413

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Harnessing the differentiation of stem cells into bone-forming cells represents an intriguing avenue for the creation of functional skeletal tissues. Therefore, a profound understanding of bone development and morphogenesis sheds light on the regenerative application of stem cells in orthopedics and dentistry. In this concise review, we summarize the studies deciphering the mechanisms that govern osteoblast differentiation in the context of in vitro formation of bone-like nodules, including morphologic and molecular events as well as cellular contributions to mineral nucleation, occurring during osteogenic differentiation of stem cells. This article also highlights the limitations of current translational applications of stem cells and opportunities to use the bone-like nodule model for bone regenerative therapies.

引用

页码：1587 / 1593

页数：7

共 65 条

[1]

Aubin JE, 2000, DRUG DEVELOP RES, V49, P206, DOI 10.1002/(SICI)1098-2299(200003)49:3<206::AID-DDR11>3.0.CO

[2]

2-G

[3] Gap junctions and hemichannels in signal transmission, function and development of bone [J].

Batra, Nidhi ;

Kar, Rekha ;

Jiang, Jean X. .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2012, 1818 (08) :1909-1918

[4] MINERALIZED BONE NODULES FORMED INVITRO FROM ENZYMATICALLY RELEASED RAT CALVARIA CELL-POPULATIONS [J].

BELLOWS, CG ;

AUBIN, JE ;

HEERSCHE, JNM ;

ANTOSZ, ME .

CALCIFIED TISSUE INTERNATIONAL, 1986, 38 (03) :143-154

[5] INITIATION AND PROGRESSION OF MINERALIZATION OF BONE NODULES FORMED INVITRO - THE ROLE OF ALKALINE-PHOSPHATASE AND ORGANIC PHOSPHATE [J].

BELLOWS, CG ;

AUBIN, JE ;

HEERSCHE, JNM .

BONE AND MINERAL, 1991, 14 (01) :27-40

[6] ULTRASTRUCTURAL ANALYSIS OF BONE NODULES FORMED INVITRO BY ISOLATED FETAL-RAT CALVARIA CELLS [J].

BHARGAVA, U ;

BARLEV, M ;

BELLOWS, CG ;

AUBIN, JE .

BONE, 1988, 9 (03) :155-163

[7]

Black Cameron R M, 2015, Curr Mol Biol Rep, V1, P132

[8] The role of intracellular calcium phosphate in osteoblast-mediated bone apatite formation [J].

Boonrungsiman, Suwimon ;

Gentleman, Eileen ;

Carzaniga, Raffaella ;

Evans, Nicholas D. ;

McComb, David W. ;

Porter, Alexandra E. ;

Stevens, Molly M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (35) :14170-14175

[9] Direct visualization of intracellular calcium in rat osteoblasts by energy-filtering transmission electron microscopy [J].

Bordat, C ;

Guerquin-Kern, JL ;

Lieberherr, M ;

Cournot, G .

HISTOCHEMISTRY AND CELL BIOLOGY, 2004, 121 (01) :31-38

[10] The importance of mineral in bone and mineral research [J].

Boyan, BD ;

Schwartz, Z ;

Boskey, AL .

BONE, 2000, 27 (03) :341-342

← 1 2 3 4 5 6 7 →