IGF-1 Protects Myocardial Cells from ROS Stress-Induced Apoptosis via Up-Regulating ARC

被引：0

作者：

Xi, Zhaofang ^{[1
]}

Zhu, Haibao ^{[1
]}

Liu, Dongyang ^{[1
]}

Wu, Liming ^{[1
]}

Guo, DingZong ^{[1
]}

机构：

[1] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China

来源：

JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2012年 / 11卷 / 11期

基金：

高等学校博士学科点专项科研基金;

关键词：

H9C2; cells; hydrogen peroxide; IGF-1; ARC; apoptosis; China; GROWTH-FACTOR-I; CASPASE RECRUITMENT DOMAIN; SKELETAL-MUSCLE; REPRESSOR; EXPRESSION; PATHWAY; REPERFUSION; ACTIVATION; INFARCTION; SURVIVAL;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Insulin-like Growth Factor-1 (IGF-1) and Apoptosis Repressor with Caspase recruitment domain (ARC) play an important role in regulating apoptosis. Although, the precise mechanisms of IGF-1 and ARC in this process have not been defined, they have similar anti-apoptotic effects in myocardial cells, suggesting that these effects are related. Researchers found that H2O2 can induce ARC reduction in H9C2 cells but IGF-1 can change this trend. To clarify this trend using immunofluorescence and immunoblot analysis, researchers found that LY294002 (PI3K inhibitor) blocked IGF-1 up-regulation of ARC protein and blocked the protective effect of IGF-1 on myocardial cell apoptosis induced by oxidative stress. These results indicate that IGF-1 up-regulates ARC protein expression via the PI3K pathway which protects against myocardial cell apoptosis induced by oxidative stress.

引用

页码：1901 / 1906

页数：6

共 25 条

[1] Characterization of ARC, apoptosis repressor interacting with CARD, in normal and dystrophin-deficient skeletal muscle [J].

Abmayr, S ;

Crawford, RW ;

Chamberlain, JS .

HUMAN MOLECULAR GENETICS, 2004, 13 (02) :213-221

[2] ARC is a critical cardiomyocyte survival switch in doxorubicin cardiotoxicity [J].

An, Junfeng ;

Li, Peifeng ;

Li, Jincheng ;

Dietz, Rainer ;

Donath, Stefan .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2009, 87 (04) :401-410

[3] Targeting the IGF-1 receptor: from rags to riches [J].

Baserga, R .

EUROPEAN JOURNAL OF CANCER, 2004, 40 (14) :2013-2015

[4] CARDIOPROTECTIVE EFFECT OF INSULIN-LIKE GROWTH-FACTOR-I IN MYOCARDIAL-ISCHEMIA FOLLOWED BY REPERFUSION [J].

BUERKE, M ;

MUROHARA, T ;

SKURK, C ;

NUSS, C ;

TOMASELLI, K ;

LEFER, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) :8031-8035

[5] Constitutive activation of MAPK cascade in acute quadriplegic myopathy [J].

Di Giovanni, S ;

Molon, A ;

Broccolini, A ;

Melcon, G ;

Mirabella, M ;

Hoffman, EP ;

Servidei, S .

ANNALS OF NEUROLOGY, 2004, 55 (02) :195-206

[6]

Ekhterae D, 1999, CIRC RES, V85, pE70

[7] Ubiquitination and degradation of the anti-apoptotic protein ARC by MDM2 [J].

Foo, Roger S. -Y. ;

Chan, Lennard K. W. ;

Kitsis, Richard N. ;

Bennett, Martin R. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (08) :5529-5535

[8] Apoptosis repressor with caspase recruitment domain (ARC) inhibits myogenic differentiation [J].

Hunter, Arwen L. ;

Zhang, Jingchun ;

Chen, Shirley C. ;

Si, Xiaoning ;

Wong, Brian ;

Ekhterae, Daryoush ;

Luo, Honglin ;

Granville, David J. .

FEBS LETTERS, 2007, 581 (05) :879-884

[9] Cardiomyocyte apoptosis is related to left ventricular dysfunction and remodelling in dilated cardiomyopathy, but is not affected by growth hormone treatment [J].

Ibe, Waltraut ;

Saraste, Antti ;

Lindemann, Stephan ;

Bruder, Stephanie ;

Buerke, Michael ;

Darius, Harald ;

Pulkki, Kari ;

Voipio-Pulkki, Liisa-Maria .

EUROPEAN JOURNAL OF HEART FAILURE, 2007, 9 (02) :160-167

[10]

KAWANO H, 1994, JPN HEART J, V35, P745

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