The bHLH Transcription Factor bHLH104 Interacts with IAA-LEUCINE RESISTANT3 and Modulates Iron Homeostasis in Arabidopsis

被引:211
作者
Zhang, Jie [1 ,2 ]
Liu, Bing [1 ,2 ]
Li, Mengshu [1 ,2 ]
Feng, Dongru [1 ,2 ]
Jin, Honglei [1 ,2 ]
Wang, Peng [1 ,2 ]
Liu, Jun [1 ,2 ]
Xiong, Feng [1 ,2 ]
Wang, Jinfa [1 ,2 ]
Wang, Hong-Bin [1 ,2 ]
机构
[1] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Sch Life Sci, Collaborat Innovat Ctr Genet & Dev, Guangdong Prov Key Lab Plant Resources, Guangzhou 510275, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
DEFICIENCY RESPONSES; FACTOR FAMILY; HIGHER-PLANTS; POLLEN DEVELOPMENT; CHELATE REDUCTASE; METAL HOMEOSTASIS; GENE-EXPRESSION; ORYZA-SATIVA; GENOME-WIDE; PROTEIN;
D O I
10.1105/tpc.114.132704
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Iron (Fe) is an indispensable micronutrient for plant growth and development. The regulation of Fe homeostasis in plants is complex and involves a number of transcription factors. Here, we demonstrate that a basic helix-loop-helix (bHLH) transcription factor, bHLH104, belonging to the IVc subgroup of bHLH family, acts as a key component positively regulating Fe deficiency responses. Knockout of bHLH104 in Arabidopsis thaliana greatly reduced tolerance to Fe deficiency, whereas overexpression of bHLH104 had the opposite effect and led to accumulation of excess Fe in soil-grown conditions. The activation of Fe deficiency-inducible genes was substantially suppressed by loss of bHLH104. Further investigation showed that bHLH104 interacted with another IVc subgroup bHLH protein, IAA-LEUCINE RESISTANT3 (ILR3), which also plays an important role in Fe homeostasis. Moreover, bHLH104 and ILR3 could bind directly to the promoters of Ib subgroup bHLH genes and POPEYE (PYE) functioning in the regulation of Fe deficiency responses. Interestingly, genetic analysis showed that loss of bHLH104 could decrease the tolerance to Fe deficiency conferred by the lesion of BRUTUS, which encodes an E3 ligase and interacts with bHLH104. Collectively, our data support that bHLH104 and ILR3 play pivotal roles in the regulation of Fe deficiency responses via targeting Ib subgroup bHLH genes and PYE expression.
引用
收藏
页码:787 / 805
页数:19
相关论文
共 69 条
  • [11] PHYSIOLOGICAL CHARACTERIZATION OF A SINGLE-GENE MUTANT OF PISUM-SATIVUM EXHIBITING EXCESS IRON ACCUMULATION .1. ROOT IRON REDUCTION AND IRON UPTAKE
    GRUSAK, MA
    WELCH, RM
    KOCHIAN, LV
    [J]. PLANT PHYSIOLOGY, 1990, 93 (03) : 976 - 981
  • [12] IRON - NUTRITIOUS, NOXIOUS, AND NOT READILY AVAILABLE
    GUERINOT, ML
    YI, Y
    [J]. PLANT PHYSIOLOGY, 1994, 104 (03) : 815 - 820
  • [13] MicroRNA mediated regulation of metal toxicity in plants: present status and future perspectives
    Gupta, O. P.
    Sharma, P.
    Gupta, R. K.
    Sharma, I.
    [J]. PLANT MOLECULAR BIOLOGY, 2014, 84 (1-2) : 1 - 18
  • [14] Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl)
    Haensch, Robert
    Mendel, Ralf R.
    [J]. CURRENT OPINION IN PLANT BIOLOGY, 2009, 12 (03) : 259 - 266
  • [15] A novel major facilitator superfamily protein at the tonoplast influences zinc tolerance and accumulation in Arabidopsis
    Haydon, Michael J.
    Cobbett, Christopher S.
    [J]. PLANT PHYSIOLOGY, 2007, 143 (04) : 1705 - 1719
  • [16] The basic helix-loop-helix transcription factor family in plants: A genome-wide study of protein structure and functional diversity
    Heim, MA
    Jakoby, M
    Werber, M
    Martin, C
    Weisshaar, B
    Bailey, PC
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 2003, 20 (05) : 735 - 747
  • [17] Iron uptake, trafficking and homeostasis in plants
    Hell, R
    Stephan, UW
    [J]. PLANTA, 2003, 216 (04) : 541 - 551
  • [18] The involvement of a multicopper oxidase in iron uptake by the green algae Chlamydomonas reinhardtii
    Herbik, A
    Bölling, C
    Buckhout, TJ
    [J]. PLANT PHYSIOLOGY, 2002, 130 (04) : 2039 - 2048
  • [19] Getting a sense for signals: Regulation of the plant iron deficiency response
    Hindt, Maria N.
    Guerinot, Mary Lou
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2012, 1823 (09): : 1521 - 1530
  • [20] Fitting into the Harsh Reality: Regulation of Iron-deficiency Responses in Dicotyledonous Plants
    Ivanov, Rumen
    Brumbarova, Tzvetina
    Bauer, Petra
    [J]. MOLECULAR PLANT, 2012, 5 (01) : 27 - 42