Long-term single-cell imaging of mammalian stem cells

被引:0
作者
Schroeder, Timm [1 ]
机构
[1] Helmholtz Zentrum Munich, German Res Ctr Environm Hlth GmbH, Inst Stem Cell Res, Neuherberg, Germany
关键词
HEMATOPOIETIC STEM; SELF-RENEWAL; FATE; GENERATION; DIFFERENTIATION; RECONSTITUTION; HETEROGENEITY; NEURONS;
D O I
10.1038/NMETH.1577
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Continuous long-term single-cell observation provides insight into the molecular control of cell fate. This is particularly important for rare and heterogeneous populations of cells, such as mammalian stem cells. The current lack of usable off-the-shelf hardware and software for such experiments makes their implementation technically challenging. Here I discuss the need for continuous single-cell quantification to understand molecular cell fate control as well as organizational and technical solutions for long-term imaging and tracking of stem cells.
引用
收藏
页码:S30 / S35
页数:6
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