Validation of different measures of insulin sensitivity of glucose metabolism in dairy cows using the hyperinsulinemic euglycemic clamp test as the gold standard

被引：33

作者：

De Koster, J. ^{[1
]}

Hostens, M. ^{[1
]}

Hermans, K. ^{[1
]}

Van den Broeck, W. ^{[2
]}

Opsomer, G. ^{[1
]}

机构：

[1] Univ Ghent, Fac Vet Med, Dept Reprod Obstet & Herd Hlth, Salisburylaan 133, B-9820 Merelbeke, Belgium

[2] Univ Ghent, Fac Vet Med, Dept Morphol, Salisburylaan 133, B-9820 Merelbeke, Belgium

来源：

DOMESTIC ANIMAL ENDOCRINOLOGY | 2016年 / 57卷

关键词：

Dairy cow; Measures; Insulin sensitivity; Insulin resistance; Hyperinsulinemic euglycemic clamp test; NONESTERIFIED FATTY-ACIDS; PREPARTUM 2,4-THIAZOLIDINEDIONE; ABOMASAL INFUSION; TOLERANCE TEST; HOLSTEIN COWS; DRY PERIOD; RESISTANCE; PLASMA; RESPONSES; MODEL;

D O I：

10.1016/j.domaniend.2016.06.004

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of the present research was to compare different measures of insulin sensitivity in dairy cows at the end of the dry period. To do so, 10 clinically healthy dairy cows with a varying body condition score were selected. By performing hyperinsulinemic euglycemic clamp (HEC) tests, we previously demonstrated a negative association between the insulin sensitivity and insulin responsiveness of glucose metabolism and the body condition score of these animals. In the same animals, other measures of insulin sensitivity were determined and the correlation with the HEC test, which is considered as the gold standard, was calculated. Measures derived from the intravenous glucose tolerance test (IVGTT) are based on the disappearance of glucose after an intravenous glucose bolus. Glucose concentrations during the IVGTT were used to calculate the area under the curve of glucose and the clearance rate of glucose. In addition, glucose and insulin data from the IVGTT were fitted in the minimal model to derive the insulin sensitivity parameter, Si. Based on blood samples taken before the start of the IVGTT, basal concentrations of glucose, insulin, NEFA, and beta-hydroxybutyrate were determined and used to calculate surrogate indices for insulin sensitivity, such as the homeostasis model of insulin resistance, the quantitative insulin sensitivity check index, the revised quantitative insulin sensitivity check index and the revised quantitative insulin sensitivity check index including beta-hydroxybutyrate. Correlation analysis revealed no association between the results obtained by the HEC test and any of the surrogate indices for insulin sensitivity. For the measures derived from the IVGTT, the area under the curve for the first 60 min of the test and the Si derived from the minimal model demonstrated good correlation with the gold standard. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：117 / 126

页数：10

共 47 条

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