High association of Cryptosporidium spp. infection with colon adenocarcinoma in Lebanese patients

被引:40
作者
Osman, Marwan [1 ,2 ]
Benamrouz, Sadia [1 ,3 ]
Guyot, Karine [1 ]
Baydoun, Martha [1 ,4 ,5 ]
Frealle, Emilie [1 ,6 ,7 ,8 ]
Chabe, Magali [1 ,9 ]
Gantois, Nausicaa [1 ]
Delaire, Baptiste [10 ]
Goffard, Anne [11 ]
Aoun, Albert [12 ]
Jurdi, Nawaf [12 ]
Dabboussi, Fouad [2 ]
Even, Gael [13 ]
Slomianny, Christian [14 ]
Gosset, Pierre [10 ]
Hamze, Monzer [2 ]
Creusy, Colette [10 ]
Viscogliosi, Eric [1 ]
Certad, Gabriela [1 ,15 ]
机构
[1] Univ Lille, CHU Lille, BDPEE, UMR CNRS 8204,INSERM U1019,Inst Pasteur Lille,CII, Lille, France
[2] Univ Libanaise, Fac Sante Publ, Ecole Doctorale Sci & Technol, LMSE, Tripoli, Lebanon
[3] Univ Catholique Lille, FGES, Ecol & Biodiversite, Lille, France
[4] Univ Lille, CNRS, ISEN, IEMN,BioMEMS,UMR 8520, Lille, France
[5] BioGAP, Grp HEI ISA ISEN, Lille, France
[6] Ctr Hosp Reg, Lille, France
[7] Univ Lille, Lille, France
[8] Univ Lille Nord France, Lab Parasitol Mycol, Ctr Biol & Pathol, Fac Med Lille, Lille, France
[9] Univ Lille Nord France, Dept Parasitol Mycol, Fac Sci Pharmaceut & Biol, Lille, France
[10] Grp Hop Univ Catholique Lille, Serv Anat & Cytol Pathol, Lille, France
[11] Univ Lille, CNRS, INSERM, Mol & Cellular Virol,Inst Pasteur Lille,CHU Lille, Lille, France
[12] Amer Univ Beirut, Fac Med, Pathol Dept, Beirut, Lebanon
[13] France PEGASE Biosci, Lille, France
[14] Univ Lille, INSERM U 1003, Lab Cell Physiol, Villeneuve Dascq, France
[15] Univ Catholique Lille, Fac Med & Maieut, Inst Catholique Lille, Dept Rech Med, Lille, France
关键词
CELL APOPTOSIS; PARVUM; PARASITES; CANCER; MODEL; TIME;
D O I
10.1371/journal.pone.0189422
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background The association between Cryptosporidium and human colon cancer has been reported in different populations. However, this association has not been well studied. In order to add new strong arguments for a probable link between cryptosporidiosis and colon human cancer, the aim of this study was to determine prevalence and to identify species of Cryptosporidium among Lebanese patients. Methodology and principal findings Overall, 218 digestive biopsies were collected in Tripoli, Lebanon, from three groups of patients: (i) patients with recently diagnosed colon intraepithelial neoplasia/adenocarcinoma before any treatment (n = 72); (ii) patients with recently diagnosed stomach intraepithelial neoplasia/adenocarcinoma before any treatment (n = 21); and (iii) patients without digestive intraepithelial neoplasia/adenocarcinoma but with persistent digestive symptoms (n = 125). DNA extraction was performed from paraffin-embedded tissue. The presence of the parasite in tissues was confirmed by PCR, microscopic observation and immunofluorescence analysis. We identified a high rate (21%) of Cryptosporidium presence in biopsies from Lebanese patients with recently diagnosed colonic neoplasia/adenocarcinoma before any treatment. This prevalence was significantly higher compared to 7% of Cryptosporidium prevalence among patients without colon neoplasia but with persistent gastrointestinal symptoms (OR: 4, CI: 1.65-9.6, P = 0.001). When the comparison was done against normal biopsies, the risk of infection increased 11-fold in the group of patients with colon adenocarcinoma (OR: 11.315, CI: 1.44-89.02, P = 0.003). Conclusions This is the first study performed in Lebanon reporting the prevalence of Cryptosporidium among patients with digestive cancer. These results show that Cryptosporidium is strongly associated with human colon cancer being maybe a potential etiological agent of this disease.
引用
收藏
页数:10
相关论文
共 25 条
[11]   Cryptosporidium parvum activates nuclear factor κB in biliary epithelia preventing epithelial cell apoptosis [J].
Chen, XM ;
Levine, SA ;
Splinter, PL ;
Tietz, PS ;
Ganong, AL ;
Jobin, C ;
Gores, GJ ;
Paya, CV ;
LaRusso, NF .
GASTROENTEROLOGY, 2001, 120 (07) :1774-1783
[12]   Systematic analysis of human oncogenic viruses in colon cancer revealed EBV latency in lymphoid infiltrates [J].
Fiorina, Loretta ;
Ricotti, Mattia ;
Vanoli, Alessandro ;
Luinetti, Ombretta ;
Dallera, Elena ;
Riboni, Roberta ;
Paolucci, Stefania ;
Brugnatelli, Silvia ;
Paulli, Marco ;
Pedrazzoli, Paolo ;
Baldanti, Fausto ;
Perfetti, Vittorio .
INFECTIOUS AGENTS AND CANCER, 2014, 9
[13]   Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study [J].
Kotloff, Karen L. ;
Nataro, James P. ;
Blackwelder, William C. ;
Nasrin, Dilruba ;
Farag, Tamer H. ;
Panchalingam, Sandra ;
Wu, Yukun ;
Sow, Samba O. ;
Sur, Dipika ;
Breiman, Robert F. ;
Faruque, Abu S. G. ;
Zaidi, Anita K. M. ;
Saha, Debasish ;
Alonso, Pedro L. ;
Tamboura, Boubou ;
Sanogo, Doh ;
Onwuchekwa, Uma ;
Manna, Byomkesh ;
Ramamurthy, Thandavarayan ;
Kanungo, Suman ;
Ochieng, John B. ;
Omore, Richard ;
Oundo, Joseph O. ;
Hossain, Anowar ;
Das, Sumon K. ;
Ahmed, Shahnawaz ;
Qureshi, Shahida ;
Quadri, Farheen ;
Adegbola, Richard A. ;
Antonio, Martin ;
Hossain, M. Jahangir ;
Akinsola, Adebayo ;
Mandomando, Inacio ;
Nhampossa, Tacilta ;
Acacio, Sozinho ;
Biswas, Kousick ;
O'Reilly, Ciara E. ;
Mintz, Eric D. ;
Berkeley, Lynette Y. ;
Muhsen, Khitam ;
Sommerfelt, Halvor ;
Robins-Browne, Roy M. ;
Levine, Myron M. .
LANCET, 2013, 382 (9888) :209-222
[14]   Hyper IgM Syndrome: a Report from the USIDNET Registry [J].
Leven, Emily A. ;
Maffucci, Patrick ;
Ochs, Hans D. ;
Scholl, Paul R. ;
Buckley, Rebecca H. ;
Fuleihan, Ramsay L. ;
Geha, Raif S. ;
Cunningham, Coleen K. ;
Bonilla, Francisco A. ;
Conley, Mary Ellen ;
Ferdman, Ronald M. ;
Hernandez-Trujillo, Vivian ;
Puck, Jennifer M. ;
Sullivan, Kathleen ;
Secord, Elizabeth A. ;
Ramesh, Manish ;
Cunningham-Rundles, Charlotte .
JOURNAL OF CLINICAL IMMUNOLOGY, 2016, 36 (05) :490-501
[15]   Biphasic Modulation of Apoptotic Pathways in Cryptosporidium parvum-Infected Human Intestinal Epithelial Cells [J].
Liu, Jin ;
Deng, Mingqi ;
Lancto, Cheryl A. ;
Abrahamsen, Mitchell S. ;
Rutherford, Mark S. ;
Enomoto, Shinichiro .
INFECTION AND IMMUNITY, 2009, 77 (02) :837-849
[16]   Multicentric Evaluation of a New Real-Time PCR Assay for Quantification of Cryptosporidium spp. and Identification of Cryptosporidium parvum and Cryptosporidium hominis [J].
Mary, C. ;
Chapey, E. ;
Dutoit, E. ;
Guyot, K. ;
Hasseine, L. ;
Jeddi, F. ;
Menotti, J. ;
Paraud, C. ;
Pomares, C. ;
Rabodonirina, M. ;
Rieux, A. ;
Derouin, F. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (08) :2556-2563
[17]   Cryptospotidium parvum at different developmental stages modulates host cell apoptosis in vitro [J].
Mele, R ;
Morales, MAG ;
Tosini, F ;
Pozio, E .
INFECTION AND IMMUNITY, 2004, 72 (10) :6061-6067
[18]   Prevalence and Risk Factors for Intestinal Protozoan Infections with Cryptosporidium, Giardia, Blastocystis and Dientamoeba among Schoolchildren in Tripoli, Lebanon [J].
Osman, Marwan ;
El Safadi, Dima ;
Cian, Amandine ;
Benamrouz, Sadia ;
Nourrisson, Celine ;
Poirier, Philippe ;
Pereira, Bruno ;
Razakandrainibe, Romy ;
Pinon, Anthony ;
Lambert, Celine ;
Wawrzyniak, Ivan ;
Dabboussi, Fouad ;
Delbac, Frederic ;
Favennec, Loic ;
Hamze, Monzer ;
Viscogliosi, Eric ;
Certad, Gabriela .
PLOS NEGLECTED TROPICAL DISEASES, 2016, 10 (03)
[19]   Initial Data on the Molecular Epidemiology of Cryptosporidiosis in Lebanon [J].
Osman, Marwan ;
El Safadi, Dima ;
Benamrouz, Sadia ;
Guyot, Karine ;
Dei-Cas, Eduardo ;
Aliouat, El Moukhtar ;
Creusy, Colette ;
Mallat, Hassan ;
Hamze, Monzer ;
Dabboussi, Fouad ;
Viscogliosi, Eric ;
Certad, Gabriela .
PLOS ONE, 2015, 10 (05)
[20]  
Shebl FM, 2012, AIDS RES HUM RETROV, V28, P794, DOI [10.1089/aid.2011.0185, 10.1089/AID.2011.0185]