Characterization of eight microsatellite loci for the sea urchin Meoma ventricosa (Spatangoida, Brissidae) through Next Generation Sequencing

被引：1
作者：
Jossart, Quentin [1 ,2 ]
Geyer, Laura B. [3 ]
Lessios, H. A. [3 ]
机构：
[1] Univ Libre Bruxelles, Biol Marine Lab, B-1050 Brussels, Belgium
[2] Univ Bourgogne, CNRS, UMR 6282, Biogeosci, F-21000 Dijon, France
[3] Smithsonian Trop Res Inst, Balboa, Panama
来源：
BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2015年 / 59卷
关键词：
Microsatellites; Echinoid; Caribbean; Tagged primer method; 454; method; ECHINOIDEA; SOFTWARE;
D O I：
10.1016/j.bse.2015.01.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Eight microsatellite loci were characterized for Meoma ventricosa (Lamarck, 1816), a burrowing sea urchin that can be afflicted by a bacterial disease causing localized mass mortality. For the analyzed population (29 individuals from St. Croix, US Virgin Islands), we observed 8.125 mean number of alleles, 0.640 mean observed heterozygosity (H-o) and 0.747 mean expected heterozygosity (H-e). Two loci showed significant deviations from Hardy-Weinberg equilibrium. Overall, the described loci were characterized by a moderately high level of polymorphism suggesting that these markers are useful for a population genetic study in the Caribbean Sea. (C) 2015 Elsevier Ltd. All. rights reserved.
引用
收藏
页码：100 / 103
页数：4
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