Effects of carbon dioxide pneumoperitoneum on hepatic and renal morphology of rats after segmental colectomy and colonic anastomosis

被引：9
作者：
Caetano Farias, Igor Eduardo [1 ,2 ]
Alves de Morais, Pedro Henrique [1 ,2 ]
Duraes, Leonardo de Castro [3 ]
Carneiro, Fabiana Pirani
de Oliveira, Paulo Goncalves
de Sousa, Joao Batista
机构：
[1] Brasilia Univ UnB, Expt Surg Lab, Sch Med, Brasilia, DF, Brazil
[2] Brasilia Univ UnB, Pathol Lab, Sch Med, Brasilia, DF, Brazil
[3] Univ Brasilia, Sch Med, Postgrad Program Med Sci, Brasilia, DF, Brazil
来源：
ACTA CIRURGICA BRASILEIRA | 2011年 / 26卷 / 04期
关键词：
Kidney; Liver; Pneumoperitoneum; Artificial; Carbon Dioxide; Laparoscopy; Rats; INCREASED INTRAABDOMINAL PRESSURE; BLOOD-FLOW; OXIDATIVE STRESS; LAPAROSCOPY; PERFUSION; ARTERIAL; IMPACT;
D O I：
10.1590/S0102-86502011000400006
中图分类号：
R61 [外科手术学];
学科分类号：
摘要：
Purpose: To evaluate the effects of increased intraperitoneal pressure caused by carbon dioxide pneumoperitoneum on the hepatic and renal morphology of rats. Methods: Fifty-four adult male rats were randomly divided into three groups (P, PP and C) after anesthesia: P - in 18 animals, pneumoperitoneum was established for 30 minutes immediately before laparotomy; PP - in 18 animals, pneumoperitoneum was established for 60 minutes divided into 30 immediately before laparotomy and 30 after abdominal closure; control group (C) - 18 animals underwent laparotomy without pneumoperitoneum induction. The pneumoperitoneum was maintained at a pressure of 5 mm Hg. Nine animals in each group were killed on the 3(rd) and 7(th) postoperative days, when kidney and liver samples were collected for morphological analysis. The liver specimens were stained with hematoxylin and eosin (HE), and the kidney specimens, with HE and von Kossa. Blinded examiners analyzed the slides. Results: No changes in renal morphology were found. Liver samples showed histological signs of degeneration in animals in the pneumoperitoneum groups killed on the 7(th) postoperative day (p=0.029). Conclusion: The CO2 pneumoperitoneum did not affect renal morphology but caused hydropic degeneration in the liver of animals killed on the 7(th) postoperative day.
引用
收藏
页码：279 / 284
页数：6
相关论文
共 25 条
[1]   Correlation of CO2 pneumoperitoneal pressures between rodents and humans [J].
Avital, Shmuel ;
Itah, Refael ;
Szomstein, Samuel ;
Rosenthal, Raul ;
Inbar, Roye ;
Sckornik, Yehuda ;
Weinbroum, Avi .
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES, 2009, 23 (01) :50-54
[2]   Power spectral analysis of heart rate variability during positive pressure pneumoperitoneum - The significance of increased cardiac sympathetic expression [J].
Bickel, A ;
Yahalom, M ;
Roguin, N ;
Frankel, R ;
Breslava, J ;
Ivry, S ;
Eitan, A .
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES, 2002, 16 (09) :1341-1344
[3]  
Bradbury Michael W, 2004, Clin Liver Dis, V8, P639, DOI 10.1016/j.cld.2004.04.005
[4]  
Chida VV, 2003, ACTA CIR BRAS, V18, P51
[5]  
CHIU AW, 1995, J AM COLL SURGEONS, V181, P397
[6]   EFFECTS OF INTRAABDOMINAL PRESSURE ON RENAL TISSUE PERFUSION DURING LAPAROSCOPY [J].
CHIU, AW ;
AZADZOI, KM ;
HATZICHRISTOU, DG ;
SIROKY, MB ;
KRANE, RJ ;
BABAYAN, RK .
JOURNAL OF ENDOUROLOGY, 1994, 8 (02) :99-103
[7]   The effect of intra-abdominal pressure on the generation of 8-iso prostaglandin F2α during laparoscopy in rabbits [J].
de Souza, AMB ;
Wang, CC ;
Chu, CY ;
Lam, PM ;
Rogers, MS .
HUMAN REPRODUCTION, 2003, 18 (10) :2181-2188
[8]   Effect of pneumoperitoneum on renal perfusion and function: A systematic review [J].
Demyttenaere, Sebastian ;
Feldman, Liane S. ;
Fried, Gerald M. .
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES, 2007, 21 (02) :152-160
[9]   EFFECT OF INCREASED INTRAABDOMINAL PRESSURE ON HEPATIC ARTERIAL, PORTAL VENOUS, AND HEPATIC MICROCIRCULATORY BLOOD-FLOW [J].
DIEBEL, LN ;
WILSON, RF ;
DULCHAVSKY, SA ;
SAXE, J ;
MEREDITH, JW ;
TIMBERLAKE, G ;
PAUL, BK ;
WITTMANN, D ;
MCSWAIN, NE ;
BERGSTEIN, JM .
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1992, 33 (02) :279-283
[10]  
Fagundes Marco Aurelio Valadao, 2001, Acta Scientiarum Universidade Estadual de Maringa, V23, P703
123