Cloning and sequencing of HPV16 e6 gene from cervical carcinoma biopsies in Xinjiang

In order to study the structure specificity of HPV16 E6 gene of a Chinese Uygur patient of cervical carcinoma in south Xinjiang, the tissue DNA was extracted from cervical carcinoma biopsies. HPV16E6 gene was amplified by PCR from the cervical carcinoma tissue DNA. The HPV16 E6 gene was cloned into pUCm-T and analyzed the whole sequence. The result of PCR showed that the positive rate of HPV16 E6 was 82.35 % (14/17). The result of sequence showed that the overall length of strain is 456 bp, it was the same as the German strain. The 247th nucleotide of HPV16 E6 mutate T to G, the mutation had changed the triplet codes, subsequently changed the amino acids coded. There is a structure difference between HPV16 E6 gene of Xinjiang strain and the standard strain.