Simultaneous quantification of oestrogens and androgens in the serum of patients with benign prostatic hyperplasia by liquid chromatography-Tandem mass spectrometry

被引：9

作者：

Yang, Tingting ^{[1
]}

Huang, Yuhan ^{[2
]}

Zhou, Yi ^{[1
]}

Chen, Shangxiu ^{[1
]}

Wang, Haiyan ^{[1
]}

Hu, Yinlu ^{[1
]}

Liu, Junjie ^{[3
]}

Jiang, Zhenzhou ^{[4
]}

Lu, Qian ^{[1
]}

Yin, Xiaoxing ^{[1
]}

机构：

[1] Xuzhou Med Univ, Jiangsu Key Lab New Drug Res & Clin Pharm, Xuzhou, Jiangsu, Peoples R China

[2] Xuzhou Med Univ, Affiliated Hosp, Dept Pharm, Xuzhou, Jiangsu, Peoples R China

[3] Xuzhou Med Univ, Affiliated Hosp, Dept Urol, Xuzhou, Jiangsu, Peoples R China

[4] China Pharmaceut Univ, Jiangsu Key Lab Drug Screening, Nanjing, Peoples R China

来源：

ANDROLOGIA | 2020年 / 52卷 / 07期

基金：

中国国家自然科学基金;

关键词：

androgens; benign prostate hyperplasia; clinical samples; hormones homeostasis; LC-MS; MS; oestrogens; DIABETIC-NEPHROPATHY; TESTOSTERONE; EXTRACT; ESTRADIOL; BLOOD; HORMONES; PATHWAY; IMPACT; RATIO;

D O I：

10.1111/and.13611

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Benign prostate hyperplasia (BPH) is a common disease in elderly men. It has been found that the occurrence of BPH was closely related to dysregulated steroid hormones. Here, a rapid, sensitive, accurate and specific method for the quantitative profiling of five androgens in man serum was developed and validated by the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Using this method, dehydroepiandrosterone (DHEA), androstenedione (A4), testosterone (T), androsterone (A), dihydrotestosterone (DHT), oestrone (E1) and oestradiol (E2) were quantified in serum from man with and without BPH. BPH patients were characterised by the decreases in DHEA, A4 and T as well as increases in DHT, E2 and E1 in serum. Meanwhile, DHEA and DHT in serum were screened as sensitive biomarkers of BPH patients. This study will provide a new perspective of dysregulated steroid hormones for the diagnosis and prevention of BPH.

引用

页数：11

共 50 条

[41] Development and validation of LC-ESI-MS/MS methods for quantification of 27 free and conjugated estrogen-related metabolites [J].

van der Berg, Carien ;

Venter, Gerda ;

van der Westhuizen, Francois H. ;

Erasmus, Elardus .

ANALYTICAL BIOCHEMISTRY, 2020, 590

[42] Steroid profiles by liquid chromatography-mass spectrometry of matched serum and single dominant ovarian follicular fluid from women undergoing IVF [J].

Walters, Kirsty A. ;

Eid, Stephanie ;

Edwards, Melissa C. ;

Thuis-Watson, Rachel ;

Desai, Reena ;

Bowman, Mark ;

Marren, Anthony J. ;

Handelsman, David J. .

REPRODUCTIVE BIOMEDICINE ONLINE, 2019, 38 (01) :30-37

[43] Metabolomics Study of Metabolic Changes in Renal Cells in Response to High-Glucose Exposure Based on Liquid or Gas Chromatography Coupled With Mass Spectrometry [J].

Wang, Liang ;

Dui, Yan ;

Xu, Bing-ju ;

Deng, Xu ;

Liu, Qing-hua ;

Zhong, Qiao-qiao ;

Wang, Chen-xiang ;

Ji, Shuai ;

Guo, Meng-zhe ;

Tang, Dao-quan .

FRONTIERS IN PHARMACOLOGY, 2019, 10

[44] An endocrine pathway in the prostate, ERβ, AR, 5α-androstane-3β,17β-diol, and CYP7B1, regulates prostate growth [J].

Weihua, Z ;

Lathe, R ;

Warner, M ;

Gustafsson, JÅ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13589-13594

[45] Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2 synthase expression in adult rats [J].

Wu, Jianhui ;

Huang, Dongyan ;

Su, Xin ;

Yan, Han ;

Sun, Zuyue .

TOXICOLOGY AND INDUSTRIAL HEALTH, 2016, 32 (11) :1848-1858

[46] Quantitative measurement of endogenous estrogens and estrogen metabolites in human serum by liquid chromatography-tandem mass spectrometry [J].

Xu, Xia ;

Roman, John M. ;

Issaq, Haleem J. ;

Keefer, Larry K. ;

Veenstra, Timothy D. ;

Zieger, Regina G. .

ANALYTICAL CHEMISTRY, 2007, 79 (20) :7813-7821

[47] Role of GPR30 in estrogen-induced prostate epithelial apoptosis and benign prostatic hyperplasia [J].

Yang, Deng-Liang ;

Xu, Jia-Wen ;

Zhu, Jian-Guo ;

Zhang, Yi-Lin ;

Xu, Jian-Bang ;

Sun, Qing ;

Cao, Xiao-Nian ;

Zuo, Wu -Lin ;

Xu, Ruo-Shui ;

Huang, Jie-Hong ;

Jiang, Fu-Neng ;

Zhuo, Yang-Jia ;

Xiao, Bai-Quan ;

Liu, Yun-Zhong ;

Yuan, Dong-Bo ;

Sun, Zhao-Lin ;

He, Hui-Chan ;

Lun, Zhao-Rong ;

Zhong, Wei-De ;

Zhou, Wen-Liang .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 487 (03) :517-524

[48] Quantitative profiling of 19 bile acids in rat plasma, liver, bile and different intestinal section contents to investigate bile acid homeostasis and the application of temporal variation of endogenous bile acids [J].

Yang, Tingting ;

Shu, Ting ;

Liu, Guanlan ;

Mei, Huifang ;

Zhu, Xiaoyu ;

Huang, Xin ;

Zhang, Luyong ;

Jiang, Zhenzhou .

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2017, 172 :69-78

[49] Disturbance of Mammary UDP-Glucuronosyltransferase Represses Estrogen Metabolism and Exacerbates Experimental Breast Cancer [J].

Zhou, Xueyan ;

Zheng, Ziqiang ;

Xu, Chang ;

Wang, Juan ;

Min, Mengjun ;

Zhao, Yun ;

Wang, Xi ;

Gong, Yinhan ;

Yin, Jiale ;

Guo, Meng ;

Guo, Dong ;

Zheng, Junnian ;

Zhang, Bei ;

Yin, Xiaoxing .

JOURNAL OF PHARMACEUTICAL SCIENCES, 2017, 106 (08) :2152-2162

[50] Protective Effects of Lepidium meyenii (Maca) Aqueous Extract and Lycopene on Testosterone Propionate-Induced Prostatic Hyperplasia in Mice [J].

Zou, Ying ;

Aboshora, Waleed ;

Li, Jing ;

Xiao, Tiancun ;

Zhang, Lianfu .

PHYTOTHERAPY RESEARCH, 2017, 31 (08) :1192-1198

← 1 2 3 4 5 →