Simultaneous quantification of oestrogens and androgens in the serum of patients with benign prostatic hyperplasia by liquid chromatography-Tandem mass spectrometry

被引：9

作者：

Yang, Tingting ^{[1
]}

Huang, Yuhan ^{[2
]}

Zhou, Yi ^{[1
]}

Chen, Shangxiu ^{[1
]}

Wang, Haiyan ^{[1
]}

Hu, Yinlu ^{[1
]}

Liu, Junjie ^{[3
]}

Jiang, Zhenzhou ^{[4
]}

Lu, Qian ^{[1
]}

Yin, Xiaoxing ^{[1
]}

机构：

[1] Xuzhou Med Univ, Jiangsu Key Lab New Drug Res & Clin Pharm, Xuzhou, Jiangsu, Peoples R China

[2] Xuzhou Med Univ, Affiliated Hosp, Dept Pharm, Xuzhou, Jiangsu, Peoples R China

[3] Xuzhou Med Univ, Affiliated Hosp, Dept Urol, Xuzhou, Jiangsu, Peoples R China

[4] China Pharmaceut Univ, Jiangsu Key Lab Drug Screening, Nanjing, Peoples R China

来源：

ANDROLOGIA | 2020年 / 52卷 / 07期

基金：

中国国家自然科学基金;

关键词：

androgens; benign prostate hyperplasia; clinical samples; hormones homeostasis; LC-MS; MS; oestrogens; DIABETIC-NEPHROPATHY; TESTOSTERONE; EXTRACT; ESTRADIOL; BLOOD; HORMONES; PATHWAY; IMPACT; RATIO;

D O I：

10.1111/and.13611

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Benign prostate hyperplasia (BPH) is a common disease in elderly men. It has been found that the occurrence of BPH was closely related to dysregulated steroid hormones. Here, a rapid, sensitive, accurate and specific method for the quantitative profiling of five androgens in man serum was developed and validated by the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Using this method, dehydroepiandrosterone (DHEA), androstenedione (A4), testosterone (T), androsterone (A), dihydrotestosterone (DHT), oestrone (E1) and oestradiol (E2) were quantified in serum from man with and without BPH. BPH patients were characterised by the decreases in DHEA, A4 and T as well as increases in DHT, E2 and E1 in serum. Meanwhile, DHEA and DHT in serum were screened as sensitive biomarkers of BPH patients. This study will provide a new perspective of dysregulated steroid hormones for the diagnosis and prevention of BPH.

引用

页数：11

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