Cysteine-Rich Intestinal Protein 1 Silencing Inhibits Migration and Invasion in Human Colorectal Cancer

被引：17

作者：

He, Guoyang ^{[1
,2
]}

Zou, Liyuan ^{[3
]}

Zhou, Lin ^{[4
]}

Gao, Peigiong ^{[1
]}

Qian, Xinlai ^{[1
]}

Cui, Jing ^{[1
]}

机构：

[1] Xinxiang Med Univ, Dept Pathol, 601 Jinsui St, Xinxiang 453000, Henan, Peoples R China

[2] Southern Med Univ, Guangdong Prov Key Lab Mol Oncol Pathol, Guangzhou, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Affiliated Hosp 3, Prevent & Hlth Care Dept, Guangzhou, Guangdong, Peoples R China

[4] Southern Med Univ, Sch Clin Med 1, Guangzhou, Guangdong, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2017年 / 44卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Cysteine-rich intestinal protein 1; SW620; HT29; Oncogenic role; Colorectal cancer; Metastasis; GENE-EXPRESSION PROFILES; IDENTIFICATION; MARKERS; CRIP1; CDNA;

D O I：

10.1159/000485357

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Cysteine-rich intestinal protein 1 (CRIP1), a member of the LIM/double zinc finger protein family, is abnormally expressed in several tumour types. However, few data are available on the role of CRIP1 in cancer. In the present study, we aimed to investigate the expression profile and functions of CRIP1 in colorectal cancer. Methods: To examine the protein expression level of CRIP1, immunohistochemistry (IHC) was performed on 56 pairs of colon cancer tissue samples. Western blotting was performed to investigate CRIP1 protein expression in four colon cancer cell lines. The endogenous expression of CRIP1 was suppressed using short interfering RNAs (siRNAs). Cell proliferation assays were used to determine whether CRIP1 silencing affected cell proliferation. Flow cytometry analysis was used to detect cell apoptosis. The effects of silencing CRIP1 on cell migration and invasion was detected using the transwell and wound-healing assays. Results: IHC analysis showed that protein level of CRIP1 was significantly higher in tumour tissue samples than in paired non-tumour tissue samples and that the CRIP1 level was higher in metastatic tissue samples than in non-metastatic tissue samples. In addition, protein levels of CRIP1 were higher in highly metastatic colon cancer cell lines than in colon cancer cell lines with low metastasis. Further, CRIP1 silencing had no effect on cell proliferation or apoptosis in SW620 and HT29 cells. CRIP1 silencing suppressed cell migration and invasion obviously in SW620 and HT29 cells. Conclusion: The present study provides new evidence that abnormal expression of CRIP1 might be related to the degree of metastasis in colorectal cancer and that CRIP1 silencing could effectively inhibit migration and invasion during colorectal cancer development. These findings might aid the development of a biomarker for colon cancer prognosis and metastasis, and thus help to treat this common type of cancer. (C) 2017 The Author(s) Published by S.Karger AG, Basel

引用

页码：897 / 906

页数：10

共 20 条

[1]

[Anonymous], 2007, INT J BIOCH CELL BIO

[2] MALDI Imaging Identifies Prognostic Seven-Protein Signature of Novel Tissue Markers in Intestinal-Type Gastric Cancer [J].

Balluff, Benjamin ;

Rauser, Sandra ;

Meding, Stephan ;

Elsner, Mareike ;

Schoene, Cedrik ;

Feuchtinger, Annette ;

Schuhmacher, Christoph ;

Novotny, Alexander ;

Juetting, Uta ;

Maccarrone, Giuseppina ;

Sarioglu, Hakan ;

Ueffing, Marius ;

Braselmann, Herbert ;

Zitzelsberger, Horst ;

Schmid, Roland M. ;

Hoefler, Heinz ;

Ebert, Matthias P. ;

Walch, Axel .

AMERICAN JOURNAL OF PATHOLOGY, 2011, 179 (06) :2720-2729

[3]

Baumhoer D, 2011, ONCOTARGET, V2, P970

[4] Worldwide Variations in Colorectal Cancer [J].

Center, Melissa M. ;

Jemal, Ahmedin ;

Smith, Robert A. ;

Ward, Elizabeth .

CA-A CANCER JOURNAL FOR CLINICIANS, 2009, 59 (06) :366-378

[5]

Chen Y, 2003, CANCER RES, V63, P1927

[6] Annual Report to the Nation on the Status of Cancer, 1975-2006, Featuring Colorectal Cancer Trends and Impact of Interventions (Risk Factors, Screening, and Treatment) to Reduce Future Rates [J].

Edwards, Brenda K. ;

Ward, Elizabeth ;

Kohler, Betsy A. ;

Eheman, Christie ;

Zauber, Ann G. ;

Anderson, Robert N. ;

Jemal, Ahmedin ;

Schymura, Maria J. ;

Lansdorp-Vogelaar, Iris ;

Seeff, Laura C. ;

van Ballegooijen, Marjolein ;

Goede, S. Luuk ;

Ries, Lynn A. G. .

CANCER, 2010, 116 (03) :544-573

[7] Human cysteine-rich intestinal protein: cDNA cloning and expression of recombinant protein and identification in human peripheral blood mononuclear cells [J].

Khoo, C ;

Blanchard, RK ;

Sullivan, VK ;

Cousins, RJ .

PROTEIN EXPRESSION AND PURIFICATION, 1997, 9 (03) :379-387

[8] Identification of brain- and bone-specific breast cancer metastasis genes [J].

Klein, Andreas ;

Olendrowitz, Christian ;

Schmutzler, Rita ;

Hampl, Juergen ;

Schlag, Peter M. ;

Maass, Nicolai ;

Arnold, Norbert ;

Wessel, Ralf ;

Ramser, Juliane ;

Meindl, Alfons ;

Scherneck, Siegfried ;

Seitz, Susanne .

CANCER LETTERS, 2009, 276 (02) :212-220

[9] Cytoskeleton-interacting LIM-domain protein CRP1 suppresses cell proliferation and protects from stress-induced cell death [J].

Latonen, Leena ;

Jarvinen, Paivi M. ;

Laiho, Marikki .

EXPERIMENTAL CELL RESEARCH, 2008, 314 (04) :738-747

[10] iTRAQ analysis of colorectal cancer cell lines suggests Drebrin (DBN1) is overexpressed during liver metastasis [J].

Lin, Qifeng ;

Tan, Hwee Tong ;

Lim, Teck Kwang ;

Khoo, Avery ;

Lim, Kiat Hon ;

Chung, Maxey C. M. .

PROTEOMICS, 2014, 14 (11) :1434-1443

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