Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus

被引：13

作者：

Carvalho, F. S. ^{[1
]}

Wenceslau, A. A. ^{[1
]}

Teixeira, M. ^{[2
]}

Albuquerque, G. R. ^{[1
]}

机构：

[1] Univ Estadual Santa Cruz, Dept Ciencias Agr & Ambientais, Programa Posgrad Ciencia Anim, Ilheus, BA, Brazil

[2] Embrapa Caprinos & Ovinos, Sobral, CE, Brazil

来源：

GENETICS AND MOLECULAR RESEARCH | 2011年 / 10卷 / 02期

关键词：

Eimeriosis; Multiplex PCR; Broilers; POLYMERASE-CHAIN-REACTION; DOMESTIC-FOWL; AVIAN EIMERIA; PCR ASSAY; DISCRIMINATION; CHICKEN; DNA; IDENTIFICATION; ACERVULINA; PREVALENCE;

D O I：

10.4238/vol10-2gmr1043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.

引用

页码：996 / 1005

页数：10

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