Biofilm acid/base physiology and gene expression in oral bacteria

Environmental pH is one the major factors affecting the composition, biological activities, and pathogenic potential of the biofilms colonizing supragingival surfaces. In periodontal diseases, small changes in pH from the metabolism of amino acids and urea may influence the activity of proteolytic enzymes of host and bacterial origin. Still, there is a significant void in the understanding of pH-dependent gene expression in bacteria, in general, and this is of course a more acute problem when one considers there is virtually no information about gene expression in response to pH in biofilms. The development of new methods and applications of some of the techniques detailed above should help to ameliorate this situation and to generate much-needed data about the role of pH in biofilm composition, stability, and activity.