Crystallization and preliminary crystallographic analysis of the archaeal intron-encoded endonuclease I-DmoI

被引：2
作者：
Dalgaard, JZ
Silva, GH
Belfort, M
Van Roey, P
机构：
[1] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA
[2] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY 12201 USA
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1998年 / 54卷
关键词：
D O I：
10.1107/S0907444998006222
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two forms of the archaeal intron-encoded site-specific endonuclease I-DmoI, namely I-DmoIc and I-DmoIl, have been purified and crystallized. Crystals of I-DmoIc are rod-shaped and diffract to 3.0 Angstrom resolution, but further analysis was hampered by twinning. Crystals of I-DmoIl, which is a six-amino-acid C-terminal truncation of I-DmoIc, are plate shaped and belong to space group C2 with cell parameters a = 93.72, b = 37.03, c = 55.56 Angstrom, beta = 113.4 degrees, with one molecule per asymmetric unit (V-m = 2.01 Angstrom(3) Da(-1)). The crystals diffract to at least 2.3 Angstrom resolution. A complete native data set has been measured and structure determination is on-going.
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页码：1435 / 1436
页数：2
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