Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH)

被引：51

作者：

Shang, Shiying ^{[1
]}

Tan, Zhongping ^{[1
]}

Danishefsky, Samuel J. ^{[1
,2
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Bioorgan Chem Lab, Mol Pharmacol & Chem Program, New York, NY 10065 USA

[2] Columbia Univ, Dept Chem, New York, NY 10027 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 15期

关键词：

alanine ligation; desulfurization; leucine ligation; peptide; valine ligation; SOLID-PHASE SYNTHESIS; CALCIUM HOMEOSTASIS; PEPTIDE-SYNTHESIS; PROTEINS; DESULFURIZATION; POLYPEPTIDES; MECHANISM; RESIDUES; INSIGHTS; STRATEGY;

D O I：

10.1073/pnas.1103118108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The power of chemical synthesis of large cysteine-free polypeptides has been significantly enhanced through the use of nonproteogenic constructs which bear strategically placed thiol groups, enabling native chemical ligation. Central to these much expanded capabilities is the specific, radical-induced, metal-free dethiolation, which can be accomplished in aqueous medium.

引用

页码：5986 / 5989

页数：4

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