Somatic Mutations and Genetic Heterogeneity at the CDKN1B Locus in Small Intestinal Neuroendocrine Tumors

被引:57
作者
Crona, Joakim [1 ]
Gustavsson, Tobias [1 ]
Norlen, Olov [1 ]
Edfeldt, Katarina [1 ]
Akerstrom, Tobias [1 ]
Westin, Gunnar [1 ]
Hellman, Per [1 ]
Bjorklund, Peyman [1 ]
Stalberg, Peter [1 ]
机构
[1] Uppsala Univ, Dept Surg Sci, Uppsala, Sweden
关键词
Tumor Lesion; Intratumor Heterogeneity; Wild Type Tumor; Sorting Intolerant From Tolerant; CDKN1B Gene;
D O I
10.1245/s10434-014-4351-9
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background. Until recently, the genetic landscape of small intestinal neuroendocrine tumors (SI-NETs) was limited to recurrent copy number alterations, most commonly a loss on chromosome 18. Intertumor heterogeneity with nonconcordant genotype in paired primary and metastatic lesions also is described, further contributing to the difficulty of unraveling the genetic enigma of SI-NETs. A recent study analyzing 55 SI-NET exomes nominated CDKN1B (p27) as a haploinsufficient tumor suppressor gene. Methods. This study aimed to determine the frequency of CDKN1B inactivation and to investigate genotype-phenotype correlations. It investigated 362 tumors from 200 patients. All samples were resequenced for mutations in CDKN1B using automated Sanger sequencing. The expression of p27 was investigated in 12 CDKN1B mutant and nine wild type tumors. Results. Some 8.5 % (17/200) of patients had tumors with pathogenic mutations in CDKN1B including 13 insertion deletions, four nonsense variants, and one stop-loss variant. All variants with available nontumoral DNA were classified as somatic. Inter-and intratumor heterogeneity at the CDKN1B locus was detected respectively in six of ten and two of ten patients. Patients with CDKN1B mutated tumors had both heterogeneous disease presentation and diverse prognosis. Expression of the p27 protein did not correlate with CDKN1B mutation status, and no differences in the clinical characteristics between CDKN1B mutated and CDKN1B wild type tumor carriers were found. Conclusion. This study corroborates the finding of CDKN1B as a potential haplo-insufficient tumor suppressor gene characterized by inter-and intratumor heterogeneity in SI-NETs.
引用
收藏
页码:S1428 / S1435
页数:8
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