Electrochemical determination of superoxide and nitric oxide generated from biological samples

被引：33

作者：

Mesaros, S

Vankova, Z

Mesarosova, A

Tomcik, P

Grunfeld, S

机构：

[1] Slovak Univ Technol Bratislava, Dept Analyt Chem, SK-81237 Bratislava, Slovakia

[2] Slovak Univ Technol Bratislava, Dept Engn Pedag, SK-81101 Bratislava, Slovakia

[3] Oakland Univ, Dept Chem, Rochester, MI 48309 USA

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1998年 / 46卷 / 01期

关键词：

superoxide; biosensor; microelectrode; nitric oxide; cardiovascular system;

D O I：

10.1016/S0302-4598(98)00107-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biological samples from the cardiovascular system were measured by amperometric enzyme electrode for superoxide determination which was prepared by anodic polymerization of pyrrole and concomitant incorporation of superoxide dismutase (SOD) on a Pt wire in phosphate buffer solution. The amperometric response to superoxide was measured at a potential of 0.7 V to oxidize the hydrogen peroxide generated. The response time of biosensor is less than 5 s and a 15 nM detection limit was achieved. Simultaneous with superoxide production was measured nitric oxide on metalloporphyrine biosensor. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：33 / 37

页数：5

共 23 条

[1] EVIDENCE FOR GENERATION OF AN ELECTRONIC EXCITATIONS STATES) IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES AND ITS PARTICIPATION IN BACTERICIDAL ACTIVITY [J].