Effect of dienogest administration on angiogenesis and hemodynamics in a rat endometrial autograft model

被引：53
作者：
Katayama, Hiroko [1 ]
Katayama, Tomihiro [1 ]
Uematsu, Kazuhiko [1 ]
Hiratsuka, Mie [2 ]
Kiyomura, Masaki [1 ]
Shimizu, Yutaka [3 ]
Sugita, Atsuro [4 ]
Ito, Masaharu [1 ]
机构：
[1] Ehime Univ, Grad Sch Med, Dept Obstet & Gynecol, Shitsukawa Toon, Ehime 7910295, Japan
[2] Fukui Womens Clin, Matsuyama, Ehime 7900922, Japan
[3] Mochida Pharmaceut Co Ltd, Pharmaceut Res Ctr, Gotemba 4128524, Japan
[4] Ehime Univ Hosp, Div Pathol, Shitsukawa Toon, Ehime 7910295, Japan
来源：
HUMAN REPRODUCTION | 2010年 / 25卷 / 11期
关键词：
dienogest; endometriosis; angiogenesis; dorsal skinfold chamber; intravital fluorescence microscopy; DEPOT MEDROXYPROGESTERONE ACETATE; GROWTH-FACTOR; PROGESTERONE-RECEPTORS; LEUPROLIDE ACETATE; STROMAL CELLS; EXPRESSION; LESIONS; STIMULATION; INHIBITION; MANAGEMENT;
D O I：
10.1093/humrep/deq241
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
We aimed to establish an endometrial autograft model in rats that would allow for repetitive in vivo analyses of angiogenesis. Dienogest (DNG) is an orally active progestin used for the treatment of endometriosis. We investigated whether DNG would affect angiogenesis of the ectopic endometrium in our model. Mechanically isolated endometrial fragments were transplanted into dorsal skinfold chambers in rats. We analyzed the effect of DNG on angiogenesis of the ectopic endometrium on Days 0, 2, 4, 7, 10 and 14 after transplantation using intravital fluorescence microscopy. The DNG-administered group showed significant suppression of angiogenesis of endometrial autografts, as indicated by the reduced size of the microvascular network and decreased microvessel density compared with those of control animals. The newly formed microvessels of the DNG-administered group showed consistently elevated diameters and centerline red blood cell velocity was decreased. Immunohistochemistry revealed a significant reduction in the level of perivascular alpha-smooth muscle actin within endometrial grafts of the DNG-administered group. DNG inhibited angiogenesis of the ectopic endometrium, with confirmed structural changes in the microvessels.
引用
收藏
页码：2851 / 2858
页数：8
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