Method for isolation of PCR-ready genomic DNA from zebrafish tissues

被引：347

作者：

Meeker, Nathan D. ^{[1
]}

Hutchinson, Sarah A. ^{[1
]}

Ho, Linh ^{[1
]}

Trecle, Nikolaus S. ^{[1
]}

机构：

[1] Univ Utah, Huntsman Canc Inst, Salt Lake City, UT 84112 USA

来源：

BIOTECHNIQUES | 2007年 / 43卷 / 05期

关键词：

DNA - Enzyme kinetics - Fish - Genes - Tissue culture - Tissue engineering;

D O I：

10.2144/000112619

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on a previously published murine protocol. The DNA solutions are of sufficient quality to allow PCR detection of transgenes from all commonly used zebrafish tissues. In sperm, transgene amplification was successful even when diluted 1000-fold, allowing easy identification of transgenic founders. Given its speed and low cost, we anticipate that the adoption of this method will streamline DNA isolation for zebrafish research.

引用

页码：610 / +

页数：3

共 3 条

[1] In vivo tracking of T cell development, ablation, and engraftment in transgenic zebrafish
Langenau, DM
Ferrando, AA
Traver, D
Kutok, JL
Hezel, JPD
Kanki, JP
Zon, LI
Look, AT
Trede, NS
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (19) : 7369 - 7374
[2] Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT)
Truett, GE
Heeger, P
Mynatt, RL
Truett, AA
Walker, JA
Warman, ML
[J]. BIOTECHNIQUES, 2000, 29 (01) : 52 - +
[3] A simple and affordable method for high-throughput DNA extraction from animal tissues for polymerase chain reaction
Yue, GH
Orban, L
[J]. ELECTROPHORESIS, 2005, 26 (16) : 3081 - 3083

← 1 →