Agrobacterium tumefaciens-Mediated Transformation of the Vegetative Dikaryotic Mycelium of the Cultivated Mushroom Flammulina velutipes

被引：25

作者：

Okamoto, Tomomitsu ^{[1
]}

Yamada, Masato ^{[1
]}

Sekiya, Shuichi ^{[1
]}

Okuhara, Toru ^{[1
]}

Taguchi, Goro ^{[1
]}

Inatomi, Satoshi ^{[2
]}

Shimosaka, Makoto ^{[1
]}

机构：

[1] Shinshu Univ, Fac Text Sci & Technol, Div Appl Biol, Nagano 3868567, Japan

[2] Hokuto Corp, Mushroom Res Lab, Nagano 3810008, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2010年 / 74卷 / 11期

基金：

日本学术振兴会;

关键词：

Agrobacterium tumefaciens-mediated transformation; basidiomycete; Flammulina velutipes; FRUITING BODY DEVELOPMENT; FILAMENTOUS FUNGI; TOOL; DNA;

D O I：

10.1271/bbb.100398

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of Flammulina velutipes using a hygromycin B resistance gene as selectable marker. The gene coding for urogen III methyltransferase (cob) was introduced into F. velutipes dikaryotic cells. The resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in F. velutipes.

引用

页码：2327 / 2329

页数：3

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