The autoreactivity of therapeutic intravenous immunoglobulin (IVIg) preparations depends on the fractionation methods used

被引:44
作者
Djoumerska, I
Tcho'banov, A
Pashov, A
Vassilev, T
机构
[1] Bulgarian Acad Sci, Stefan Angelov Inst Microbiol, Dept Immunol, BU-1113 Sofia, Bulgaria
[2] Bulgarian Acad Sci, Inst Biol & Immunol Reprod, Lab Mol Immunol, BU-1113 Sofia, Bulgaria
关键词
D O I
10.1111/j.1365-3083.2005.01568.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Natural immunoglobulin G (IgG) autoantibodies are present in the plasma of healthy individuals and, as a result, in pooled therapeutic intravenous immunoglobulin (IVIg) preparations. The production processes of commercial lVIg preparations involve different fractionation and virus-in activation steps that include in some cases treatments at extreme conditions. Different physical and chemical treatments are known to augment greatly the reactivity of natural autoantibodies to self-antigens. It is not clear to what extent the self-reactivity of IVIg preparations is due to the presence of natural IgG antibodies in the plasma pools used for fractionation, and to what extent it is due to the treatments that the of IgG molecules have been subjected to during the fractionation process. We compared the binding of seven different commercial IVIg preparations to human liver antigens. All studied IVIg's could be clearly separated into two distinct groups: those that possess significant self-reactivity and those with low binding to self-antigens. Increased self-binding was seen in the preparations produced using a fractionation step at low pH. The treatment of lVIg at low pH resulted in increasing the inhibitory effect of the pooled IgG on PHA-induced proliferation of human peripheral blood mononuclear cells. IVIg's with high and low self-binding may have different immunomodulating activities when infused to autoimmune patients.
引用
收藏
页码:357 / 363
页数:7
相关论文
共 41 条