The cellular lipids of Romboutsia

被引:16
作者
Guan, Ziqiiang [1 ]
Chen, Lingli [2 ]
Gerritsen, Jacoline [3 ,4 ]
Smidt, Hauke [3 ]
Goldfine, Howard [2 ]
机构
[1] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
[2] Univ Penn, Perelman Sch Med, Dept Microbiol, Philadelphia, PA 19104 USA
[3] Wageningen Univ, Microbiol Lab, Wageningen, Netherlands
[4] Winclove Probiot BV, Amsterdam, Netherlands
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2016年 / 1861卷 / 09期
关键词
Romboutsia; Glycolipid; Mass spectrometry; Phospholipid; Thin-layer chromatography; CASEI DSM 20021; CLOSTRIDIUM-ACETOBUTYLICUM; LIPOTEICHOIC ACID; GENUS CLOSTRIDIUM; POLAR LIPIDS; PHOSPHOLIPIDS; GLYCOLIPIDS; RESISTANCE; MEMBRANE; PEPTIDES;
D O I
10.1016/j.bbalip.2016.06.006
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have examined the lipids of three isolates, Romboutsia lituseburensis, Romboutsia ilealis, and Romboutsia sp. strain FRIFI, of the newly described genus Romboutsia by two-dimensional thin-layer chromatography (2D-TLC) and by liquid chromatography/mass spectrometry (LC/MS). We have found three phospholipids, phosphatidylglycerol (PG), cardiolipin and phosphatidic acid in all three species. A fourth phospholipid, lysyl-PG, was found in R. lituseburensis and strain FRIFI. Polyprenyl-phosphates were identified in the lipid extracts of all three species. Three glycolipids, mono-, di- and tri-hexosyldiacylglycerol, were common to all three species. An additional glycolipid, tetrahexosyl-diacylglycerol was identified in strain FRIFI. Acylated trihexosyldiacylglycerol and acyl-tetrahexosydiacylglycerol were also found in R. ilealis and strain FRIFI. Remarkably, no alk-1-enyl ether lipids (plasmalogens) were present in Romboutsia as distinct from bacteria of the related genus Clostridium in which these ether lipids are common. We have compared the lipidome of Romboutsia with that recently described for Clostridium difficile, which has plasmalogens, no lysyl-PG, and no tetrahexosyl-diacylglycerol. According to 16S rRNA gene sequencing, Romboutsia spp. and C. difficile are closely related (>95% sequence identity). (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:1076 / 1082
页数:7
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