Activation of phosphatidylinositol 3-kinase by prolactin in Nb2 cells

被引：39

作者：

AlSakkaf, KA

Dobson, PRM

Brown, BL

机构：

[1] UNIV SHEFFIELD,SCH MED,INST ENDOCRINOL,CELL SIGNALLING GRP,DEPT HUMAN METAB & CLIN BIOCHEM,SHEFFIELD S10 2RX,S YORKSHIRE,ENGLAND

[2] UNIV SHEFFIELD,SCH MED,INST CANC STUDIES,BIOCHEM SECT,SHEFFIELD S10 2RX,S YORKSHIRE,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 221卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.0673

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present studies, using anti-phosphotyrosine (PY20) and PI3-kinase (p85) antibodies, we have shown that PRL causes activation of phosphatidyl inositol 3-kinase (PI3-kinase) in vitro in a dose- and time-dependent manner in Nb2 cells. PRL activated PI3-kinase was completely inhibited by LY294002 (1 mu g/ml). Stimulation of the cells with PRL also increased tyrosine phosphorylation of the 85-kDa regulatory subunit. Moreover, in vitro kinase assay followed by SDS-PAGE protein separation demonstrated the phosphorylation of several other proteins besides the p85. However, no direct association between p85 and JAK2 tyrosine kinase was observed. These results indicate, for the first time, the involvement of PI3-kinase in PRL-stimulated Nb2 cell growth. (C) 1996 Academic Press. Inc.

引用

页码：779 / 784

页数：6

共 27 条

[1]

ALSAKKAF KA, 1994, J ENDOCRINOL S, V140, pP191

[2] INTERLEUKIN-2 AND POLYOMAVIRUS MIDDLE T-ANTIGEN-INDUCED MODIFICATION OF PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY IN ACTIVATED LYMPHOCYTES-T [J].

AUGUSTINE, JA ;

SUTOR, SL ;

ABRAHAM, RT .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (09) :4431-4440

[3] PROLACTIN STIMULATION OF ORNITHINE DECARBOXYLASE AND MITOGENESIS IN NB-2 NODE LYMPHOMA-CELLS - THE ROLE OF PROTEIN-KINASE-C AND CALCIUM MOBILIZATION [J].

BUCKLEY, AR ;

MONTGOMERY, DW ;

KIBLER, R ;

PUTNAM, CW ;

ZUKOSKI, CF ;

GOUT, PW ;

BEER, CT ;

RUSSELL, DH .

IMMUNOPHARMACOLOGY, 1986, 12 (01) :37-51

[4] PROLACTIN-INDUCED PHOSPHORYLATION AND NUCLEAR TRANSLOCATION OF MAP KINASE IN NB2 LYMPHOMA-CELLS [J].

BUCKLEY, AR ;

RAO, YP ;

BUCKLEY, DJ ;

GOUT, PW .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 204 (03) :1158-1164

[5] ACTIVATION OF JAK2 TYROSINE KINASE BY PROLACTIN RECEPTORS IN NB-2 CELLS AND MOUSE MAMMARY-GLAND EXPLANTS [J].

CAMPBELL, GS ;

ARGETSINGER, LS ;

IHLE, JN ;

KELLY, PA ;

RILLEMA, JA ;

CARTERSU, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (12) :5232-5236

[6] STIMULATION OF RECEPTOR-ASSOCIATED KINASE, TYROSINE KINASE, AND MAP KINASE IS REQUIRED FOR PROLACTIN-MEDIATED MACROMOLECULAR BIOSYNTHESIS AND MITOGENESIS IN NB2 LYMPHOMA [J].

CAREY, GB ;

LIBERTI, JP .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1995, 316 (01) :179-189

[7] PHOSPHATIDYLINOSITOL 3-KINASE ACTIVATION IS REQUIRED FOR INSULIN STIMULATION OF PP70 S6 KINASE, DNA-SYNTHESIS, AND GLUCOSE-TRANSPORTER TRANSLOCATION [J].

CHEATHAM, B ;

VLAHOS, CJ ;

CHEATHAM, L ;

WANG, L ;

BLENIS, J ;

KAHN, CR .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (07) :4902-4911

[8] RAPAMYCIN FKBP SPECIFICALLY BLOCKS GROWTH-DEPENDENT ACTIVATION OF AND SIGNALING BY THE 70 KD S6 PROTEIN-KINASES [J].

CHUNG, J ;

KUO, CJ ;

CRABTREE, GR ;

BLENIS, J .

CELL, 1992, 69 (07) :1227-1236

[9] THE PROTEIN-TYROSINE KINASE P59(FYN) IS ASSOCIATED WITH PROLACTIN (PRL) RECEPTOR AND IS ACTIVATED BY PRL STIMULATION OF T-LYMPHOCYTES [J].

CLEVENGER, CV ;

MEDAGLIA, MV .

MOLECULAR ENDOCRINOLOGY, 1994, 8 (06) :674-681

[10]

CLEVENGER CV, 1994, J BIOL CHEM, V269, P5559

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