The Frequency of HIV-1 Infection in Iranian Children and Determination of the Transmitted Drug Resistance in Treatment-Naive Children

被引：5

作者：

Jarchi, Maryam ^{[1
]}

Bokharaei-Salim, Farah ^{[1
]}

Esghaei, Maryam ^{[1
]}

Kiani, Seyed Jalal ^{[1
]}

Jahanbakhshi, Fatemeh ^{[2
]}

Monavari, Seyed Hamidreza ^{[1
]}

Ataei-Pirkooh, Angila ^{[1
]}

Marjani, Arezoo ^{[1
]}

Keyvani, Hossein ^{[1
]}

机构：

[1] Iran Univ Med Sci, Sch Med, Dept Virol, Tehran, Iran

[2] Pasteur Inst Iran, Dept Virol, Tehran, Iran

来源：

CURRENT HIV RESEARCH | 2019年 / 17卷 / 06期

关键词：

HIV-1; antiretroviral therapy (ART); drug resistance mutations (DRMs); HIV-1-infected; Treatment-naive Children; transmitted drug resistance (TDR); ANTIRETROVIRAL TREATMENT; REVERSE-TRANSCRIPTASE; TREATMENT FAILURE; VIRAL LOAD; MUTATIONS; VIRUS; THERAPY; PREVALENCE; WOMEN; PREVENTION;

D O I：

10.2174/1570162X17666191106111211

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background: The advent of resistance-associated mutations in HIV-1 is a barrier to the success of the ARTs. Objective: In this study, the abundance of HIV-1 infection in Iranian children, and also detection of the TDR in naive HIV-1 infected pediatric (under 12 years old) were evaluated. Materials: From June 2014 to January 2019, a total of 544 consecutive treatment-naive HIV-1-infected individuals enrolled in this study. After RNA extraction, amplification, and sequencing of the HIV-1 pol gene, the DRM and phylogenetic analysis were successfully performed on the plasma specimens of the ART-naive HIV-1-infected-children under 12 years old. The DRMs were recognized using the Stanford HIV Drug Resistance Database. Results: Out of the 544 evaluated treatment-naive HIV-1-infected individuals, 15 (2.8%) cases were children under 12 years old. The phylogenetic analyses of the amplified region of pol gene indicated that all of the 15 HIV-1-infected pediatric patients were infected by CRF35(-)AD, and a total of 13.3% (2/15) of these children were infected with HIV-1 variants with SDRMs (one child harbored two related SDRMs [D67N, V179F], and another child had three related SDRMs [M184V, T215F, and K103N]), according to the last algorithm of the WHO. No PIs-related SDRMs were observed in HIV-1-infected children. Conclusion: The current study demonstrated that a total of 13.3% of treatment-naive HIV-1-infected Iranian pediatrics (under 12 years old) were infected with HIV-1 variants with SDRMs. Therefore, it seems that screening to recognize resistance-associated mutations before the initiation of ARTs among Iranian children is essential for favorable medication efficacy and dependable prognosis.

引用

页码：397 / 407

页数：11