Sapientia aquatica gen. nov., sp. nov., isolated from a crater lake

A new aerobic betaproteobacterium, strain SA-152(T), was isolated from the water of a crater lake. 16S rRNA gene sequence analysis revealed that strain SA-152(T) belonged to the family Oxalobacteraceae (order Burkholderiales) and was phylogenetically related to Solimicrobium silvestre S20-91(T) with 97.09 % and to Herminiimonas arsenicoxydans ULPAs1(T) with 96.00% 16S rRNA gene pairwise sequence similarity. Cells of strain SA-152(T) were rod-shaped, non-motile, oxidase-negative and catalase-positive. Its fatty acid profile was dominated by two fatty acids, C-16:1 omega 7c and C-16:0, the major respiratory quinones were Q-8 and Q-7, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain SA-152(T) was 48.3 mol%. The new bacterium can be distinguished from closely related genera Solimicrobium, Herminiimonas, Rugamonas and Undibacterium based on its non-motile and oxidase-negative cells. On the basis of the phenotypic, chemotaxonomic and genomic data, strain SA-152(T) is considered to represent a novel species of a new genus, for which the name Sapientia aquatica gen. nov., sp. nov. is proposed. The type strain of Sapientia aquatica is SA-152(T) (=DSM 29805(T)=NCAIM B.02613(T)).