Detection of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli in Faecal Samples of Healthy Mithun (Bos frontalis) by Multiplex Polymerase Chain Reaction

被引：5

作者：

Rajkhowa, S. ^{[1
]}

Das, R. ^{[2
]}

Bora, S. ^{[2
]}

Rajkhowa, C. ^{[2
]}

Rahman, H. ^{[3
]}

Bujarbaruah, K. M. ^{[4
]}

机构：

[1] ICAR Res Complex, Natl Res Ctr Pig, Gauhati, Assam, India

[2] ICAR Res Complex, Jharnapani, Nagaland, India

[3] ICAR Complex NEH Reg, Sikkim, India

[4] ICAR Res Complex, New Delhi, India

来源：

ZOONOSES AND PUBLIC HEALTH | 2010年 / 57卷 / 06期

关键词：

EPEC; mithun; multiplex-PCR; STEC; CATTLE; STEC; PREVALENCE; VIRULENCE; CALCUTTA; STRAINS; EAE; ASSOCIATION; SEROTYPES; DIARRHEA;

D O I：

10.1111/j.1863-2378.2009.01249.x

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

P>Faecal samples obtained from 190 healthy mithuns were examined for the presence of Escherichia coli. Total one-hundred and five E. coli isolates were obtained from these samples, which belonged to 55 different serogroups. These isolates were subjected to multiplex polymerase chain reaction (m-PCR) for detection of stx1, stx2, eaeA and hlyA genes. Twenty-three (21.90%) E. coli isolates belonging to 14 serogroups revealed the presence of at least one virulence gene when examined by m-PCR. Nineteen percent and 2.85% of the mithuns were found to carry Shiga toxin-producing E. coli (STEC) and enteropathogenic E. coli, respectively. stx1 and stx2 genes were found to be prevalent in 7 (6.67%) and 18 (17.14%) of the isolates respectively, whereas eaeA and hlyA genes were found to be carried by three (2.85% each) isolates. Interestingly, none of the STEC isolates belonged to serogroup O157.

引用

页码：397 / 401

页数：5

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