A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle

被引：19

作者：

Chiba, Eriko ^{[1
]}

Villena, Julio ^{[1
]}

Hosoya, Shoichi ^{[1
]}

Takanashi, Naoya ^{[1
]}

Shimazu, Tomoyuki ^{[2
]}

Aso, Hisashi ^{[3
]}

Tohno, Masanori ^{[4
]}

Suda, Yoshihito ^{[5
]}

Kawai, Yasushi ^{[1
]}

Saito, Tadao ^{[1
]}

Miyazawa, Kenji ^{[6
]}

He, Fang ^{[6
]}

Kitazawa, Haruki ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Prod Chem, Food Immunol Grp, Sendai, Miyagi 9818555, Japan

[2] Tohoku Univ, Grad Sch Agr Sci, Lab Anim Breeding & Genet, Sendai, Miyagi 9818555, Japan

[3] Tohoku Univ, Grad Sch Agr Sci, Cell Biol Lab, Sendai, Miyagi 9818555, Japan

[4] Natl Inst Livestock & Grassland Sci, Natl Agr & Food Res Org, Nasushiobara 3292793, Japan

[5] Miyagi Univ, Dept Food Agr & Environm, Sendai, Miyagi 9820215, Japan

[6] Takanashi Milk Prod Co Ltd, Tech Res Lab, Yokohama, Kanagawa 2410023, Japan

来源：

RESEARCH IN VETERINARY SCIENCE | 2012年 / 93卷 / 02期

基金：

日本学术振兴会;

关键词：

Antiviral immune response; Bovine intestinal epithelial cells; Immunobiotic; Toll-like receptor 3 (TLR3); TOLL-LIKE RECEPTOR-3; MOLECULAR IMMUNOASSAY SYSTEM; INNATE IMMUNE-RESPONSES; DOUBLE-STRANDED-RNA; ROTAVIRUS INFECTION; DENDRITIC CELLS; EXPRESSION; TLR3; PROBIOTICS; CYTOKINES;

D O I：

10.1016/j.rvsc.2011.10.002

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We evaluated whether a bovine intestinal epithelial (BIE) cell line could serve as a useful in vitro model system for studying antiviral immune responses in bovine intestinal epithelial cells (IECs) and for the primary screening of immunobiotic microorganisms with antiviral protective capabilities. Immunofluorescent analyses revealed that toll-like receptor 3 (TLR3) was expressed in BIE cells, and the results of real-time quantitative PCR showed that these cells respond to stimulation with poly(I:C) by up-regulating pro-inflammatory cytokines and type I interferons. In addition, we demonstrated that BIE cells are useful for the primary screening of immunobiotic lactic acid bacteria strains which are able to beneficially modulate antiviral immune responses triggered by TLR3 activation in bovine IECs. The characterization of BIE cells performed in the present study represents an important step towards the establishment of a valuable bovine in vitro system that could be used for the development of immunomodulatory feed for bovine hosts. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：688 / 694

页数：7

共 25 条

[1] Comparative analysis of innate immune responses following infection of newborn calves with bovine rotavirus and bovine coronavirus [J].