Bead-beating artefacts in the Bacteroidetes to Firmicutes ratio of the human stool metagenome

被引：16

作者：

Vebo, Heidi C. ^{[1
,3
]}

Karlsson, Magdalena Kauczynska ^{[1
]}

Avershina, Ekaterina ^{[2
]}

Finnby, Lene ^{[1
]}

Rudi, Knut ^{[2
]}

机构：

[1] Genet Anal AS, Oslo, Norway

[2] Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, As, Norway

[3] Gentian AS, Moss, Norway

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2016年 / 129卷

关键词：

Metagenome; Human; Bead-beating; GUT MICROBIOTA;

D O I：

10.1016/j.mimet.2016.08.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We evaluated bead-beating cell-lysis in analysing the human stool metagenome, since this is a key step. We observed that two different bead-beating instruments from the same producer gave a three-fold difference in the Bacteroidetes to Firmicutes ratio. This illustrates that bead-beating can have a major impact on downstream metagenome analyses. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：78 / 80

页数：3

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