Immortalization of Primary Keratinocytes and Its Application to Skin Research

被引：33

作者：

Choi, Moonju ^{[1
]}

Lee, Choongho ^{[1
]}

机构：

[1] Dongguk Univ Seoul, Coll Pharm, Goyang 410820, South Korea

来源：

BIOMOLECULES & THERAPEUTICS | 2015年 / 23卷 / 05期

关键词：

Primary keratinocyte; Lifespan; Senescence; Immortalization; Skin research; Three-dimensional skin culture; HUMAN-PAPILLOMAVIRUS TYPE-16; MAMMARY EPITHELIAL-CELLS; TELOMERASE ACTIVITY; REPLICATIVE SENESCENCE; CELLULAR SENESCENCE; LIFE-SPAN; EFFICIENT IMMORTALIZATION; HUMAN FIBROBLASTS; NORMAL GROWTH; CPG ISLAND;

D O I：

10.4062/biomolther.2015.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As a major component of the epidermal tissue, a primary keratinocyte has served as an essential tool not only for the study of pathogenesis of skin-related diseases but also for the assessment of potential toxicities of various chemicals used in cosmetics. However, its short lifespan in ex vivo setting has been a great hurdle for many practical applications. Therefore, a number of immortalization attempts have been made with success to overcome this limitation. In order to understand the immortalization process of a primary keratinocyte, several key biological phenomena governing its lifespan will be reviewed first. Then, various immortalization methods for the establishment of stable keratinocyte cell lines will be explained. Finally, its application to a three-dimensional skin culture system will be described.

引用

页码：391 / 399

页数：9

共 79 条

[21]

DURST M, 1987, ONCOGENE, V1, P251

[22] Establishment of the human papillomavirus type 16 (HPV-16) life cycle in an immortalized human foreskin keratinocyte cell line [J].

Flores, ER ;

Allen-Hoffmann, BL ;

Lee, D ;

Sattler, CA ;

Lambert, PF .

VIROLOGY, 1999, 262 (02) :344-354

[23]

Foster SA, 1996, ONCOGENE, V12, P1773

[24] Inactivation of p16 in human mammary epithelial cells by CpG island methylation [J].

Foster, SA ;

Wong, DJ ;

Barrett, MT ;

Galloway, DA .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (04) :1793-1801

[25] Critical pathways in cellular senescence and immortalization revealed by gene expression profiling [J].

Fridman, A. L. ;

Tainsky, M. A. .

ONCOGENE, 2008, 27 (46) :5975-5987

[26] Expression profiling identifies three pathways altered in cellular immortalization: Interferon, cell cycle, and cytoskeleton [J].

Fridman, Aviva Levine ;

Tang, Lin ;

Kulaeva, Olga I. ;

Ye, Bin ;

Li, Qunfang ;

Nahhas, Fatimah ;

Roberts, Paul C. ;

Land, Susan J. ;

Abrams, Judith ;

Tainsky, Michael A. .

JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 2006, 61 (09) :879-889

[27] Telomeres, telomerase and cancer [J].

Greider, CW ;

Blackburn, EH .

SCIENTIFIC AMERICAN, 1996, 274 (02) :92-97

[28] Efficient immortalization of primary human cells by p16INK4a-specific short hairpin RNA or Bmi-1, combined with introduction of hTERT [J].

Haga, Kei ;

Ohno, Shin-ichi ;

Yugawa, Takashi ;

Narisawa-Saito, Mako ;

Fujita, Masatoshi ;

Sakamoto, Michiie ;

Galloway, Denise A. ;

Kiyono, Tohru .

CANCER SCIENCE, 2007, 98 (02) :147-154

[29] Telomerase is not an oncogene [J].

Harley, CB .

ONCOGENE, 2002, 21 (04) :494-502

[30] HPV16 E6-PROTEINS AND E7-PROTEINS COOPERATE TO IMMORTALIZE HUMAN FORESKIN KERATINOCYTES [J].

HAWLEYNELSON, P ;

VOUSDEN, KH ;

HUBBERT, NL ;

LOWY, DR ;

SCHILLER, JT .

EMBO JOURNAL, 1989, 8 (12) :3905-3910

← 1 2 3 4 5 6 7 8 →