First isolation and molecular characterization of Ehrlichia canis in Costa Rica, Central America

被引：26

作者：

Romero, L. E. ^{[1
,2
]}

Meneses, A. I. ^{[3
]}

Salazar, L. ^{[4
]}

Jimenez, M. ^{[5
]}

Romero, J. J. ^{[2
,6
]}

Aguiar, D. M. ^{[7
]}

Labruna, M. B. ^{[8
]}

Dolz, G. ^{[2
,6
]}

机构：

[1] MAG, Lab Cent Diagnost Vet, San Salvador, El Salvador

[2] Univ Nacl UNA, Escuela Med Vet, Posgrad Reg Ciencias Vet Trop PCVET, Heredia, Costa Rica

[3] UNA, Escuela Med Vet, Lab Anal Clin, Heredia, Costa Rica

[4] Univ Costa Rica, Ctr Invest Hematol & Trastornos Afines CIHATA, San Jose, Costa Rica

[5] UNA, Escuela Med Vet, Hosp Especies Menores, Heredia, Costa Rica

[6] UNA, Escuela Med Vet, Programa Invest Med Poblac MEDPOB, Heredia, Costa Rica

[7] Univ Fed Mato Grosso, Fac Agron & Med Vet, Dept Clin Med Vet, Cuiaba, MT, Brazil

[8] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo, Brazil

来源：

RESEARCH IN VETERINARY SCIENCE | 2011年 / 91卷 / 01期

关键词：

Ehrlichia canis; Isolation; PCR; Costa Rica; Dogs; POLYMERASE-CHAIN-REACTION; ETIOLOGIC AGENT; DOGS; CHAFFEENSIS; INFECTION; EWINGII; TICKS; ANAPLASMATACEAE; RICKETTSIALES; EQUI;

D O I：

10.1016/j.rvsc.2010.07.021

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The present study investigated Ehrlichia species in blood samples from dogs suspected of clinical ehrlichiosis, using molecular and isolation techniques in cell culture. From a total of 310 canine blood samples analyzed by 16S rRNA nested PCR, 148 (47.7%) were positive for Ehrlichia canis. DNA from Ehrlichia chaffeensis or Ehrlichia ewingii was not detected in any sample using species-specific primers in separated reactions. Leukocytes from five PCR-positive dogs were inoculated into DH82 cells; successful isolation of E. canis was obtained in four samples. Partial sequence of the dsb gene of eight canine blood samples (including the five samples for in vitro isolation) was obtained by PCR and their analyses through BLAST showed 100% of identity with the corresponding sequence of E. canis in GenBank. This study represents the first molecular diagnosis, isolation, and molecular characterization of E. canis in dogs from Costa Rica. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：95 / 97

页数：3

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