A label-free electrochemical immunosensor for CA125 detection based on CMK-3(Au/Fc@MgAl-LDH)n multilayer nanocomposites modification

被引：34

作者：

Wu, Mengdie ^{[1
,2
]}

Liu, Simin ^{[1
,3
]}

Qi, Feifan ^{[1
,3
]}

Qiu, Ren ^{[1
,3
]}

Feng, Jing ^{[4
]}

Ren, Xinshui ^{[1
,2
]}

Rong, Shengzhong ^{[6
]}

Ma, Hongkun ^{[6
]}

Chang, Dong ^{[5
]}

Pan, Hongzhi ^{[1
]}

机构：

[1] Shanghai Univ Med & Hlth Sci, Collaborat Res Ctr, Shanghai 201318, Peoples R China

[2] Shanghai Univ Tradit Chinese Med, Shanghai Univ Med & Hlth Sci, Shanghai 200093, Peoples R China

[3] Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

[4] Shanghai Univ Med & Hlth Sci, Coll Med Technol, Shanghai 201318, Peoples R China

[5] Fudan Univ, Affiliated Pudong Hosp, Dept Clin Lab, Shanghai 201399, Peoples R China

[6] Mudanjiang Med Univ, Sch Publ Hlth, Mudanjiang 157011, Peoples R China

来源：

TALANTA | 2022年 / 241卷

基金：

中国国家自然科学基金;

关键词：

Cancer antigen 125; Ordered mesoporous carbon; Ferrocenecarboxylic acid; Layer-by-layer assembly; Layered double hydroxides;

D O I：

10.1016/j.talanta.2022.123254

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A label-free electrochemical immunosensor was constructed for cancer antigen 125 (CA125) detection based on multiple-enlargement means of layer-by-layer (LBL) assembly of ordered mesoporous carbon (CMK-3), gold nanoparticles (Au NPs) and MgAl layered double hydroxides containing ferrocenecarboxylic acid (Fc@MgAl-LDH). A CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was designed using technology of LBL self-assembly among negatively charged Au NPs, positively charged CMK-3 and Fc@MgAl-LDH nanosheets. The CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was used as carriers to increase the immobilization of antibody and the number of loading Fc, conductors to strengthen conductivity and enhancers to amplify signal of Fc step-by-step. Besides, this special and excellent way of LBL assembly can immensely amplify the signal of immunosensor and more immobilize the biomolecules, and label-free method is a more simple the measuring way and the procedure. The immunosensor displayed a wider linear range of 0.01 U ml 1-1000 U ml(-1) and a lower detection limit of 0.004 U ml(-1). Therefore, the sensor can stablely and accurately be applied for CA125 detection in clinical cancer diagnosis.

引用

页数：7

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