Deep Sequencing to Detect Diversi of Trypanosoma cruzi Infection in Patients Coinfected With Human Immunodeficiency Virus and Chagas Disease

被引：5

作者：

Bowman, Natalie M. ^{[1
]}

Balasubramanian, Sujata ^{[2
,8
]}

Gilman, Robert H. ^{[3
]}

Parobek, Christian ^{[2
,9
]}

Calderon, Maritza ^{[4
]}

Waltmann, Andreea ^{[5
]}

Messenger, Louisa A. ^{[6
]}

Sanchez, Leny ^{[4
,10
]}

Bern, Caryn ^{[7
]}

Juliano, Jonathan J. ^{[1
]}

机构：

[1] Univ North Carolina Chapel Hill, Sch Med, Dept Med, Div Infect Dis, Chapel Hill, NC USA

[2] Univ North Carolina Chapel Hill, Sch Med, Chapel Hill, NC USA

[3] Johns Hopkins Bloomberg Sch Publ Hlth, Dept Int Hlth, Baltimore, MD USA

[4] Univ Peruana Cayetano Heredia, Lima, Peru

[5] Univ North Carolina Chapel Hill, Inst Global Hlth & Infect Dis, Chapel Hill, NC USA

[6] London Sch Hyg & Trop Med, Dept Dis Control, London, England

[7] Univ Calif San Francisco, Sch Med, Dept Epidemiol & Biostat, San Francisco, CA USA

[8] Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX USA

[9] Brown Univ, Women & Infants Hosp, Dept Obstet & Gynecol, Providence, RI USA

[10] Inst Nacl Salud, Lab VIH, Lima, Peru

来源：

JOURNAL OF INFECTIOUS DISEASES | 2022年 / 225卷 / 02期

关键词：

Trypanosoma cruzi; Chagas disease; HIV; genetic diversity; discrete typing unit; multiclonality; amplicon deep sequencing; tcsc5d;

D O I：

10.1093/infdis/jiab350

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Chagas disease, caused by Trypanosoma cruzi, can reactivate and cause severe acute disease in immunocompromised patients such as those infected with human immunodeficiency virus (HIV). We conducted amplicon deep sequencing of a 327-bp fragment of the tcscd5 gene using an Ion Torrent PGM directly from clinical samples from HIV patients with high parasitemia. We describe the within-host diversity, both characterizing the discrete typing unit of the infections and confirming the presence of multistrain infections, directly from clinical samples. This method can rapidly provide information on the genetic diversity of T. cruzi infection, which can have direct impacts on clinical disease.

引用

页码：243 / 247

页数：5

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