Curvulin and spirostaphylotrichins R and U from extracts produced by two endophytic Bipolaris sp. associated to aquatic macrophytes with antileishmanial activity

被引:15
作者
de Almeida, Tiago Tognolli [1 ,2 ]
dos Santos Ribeiro, Marcos Alessandro [1 ,3 ]
Polonio, Julio Cesar [1 ]
Garcia, Francielle Pelegrin [4 ]
Nakamura, Celso Vataru [4 ]
Meurer, Eduardo Cesar [5 ]
Sarragiotto, Maria Helena [3 ]
Baldoqui, Debora Cristina [3 ]
Azevedo, Joao Lucio [6 ]
Pamphile, Joao Alencar [1 ]
机构
[1] Univ Estadual Maringa, Dept Biotecnol Genet & Biol Celular, Maringa, Parana, Brazil
[2] Univ Sao Paulo, CENA, Piracicaba, Brazil
[3] Univ Estadual Maringa, Dept Quim, Maringa, Parana, Brazil
[4] Univ Estadual Maringa, Lab Inovacao Tecnol Desenvolvimento Farmacos & Co, Programa Posgrad Ciencias Biol, Maringa, Parana, Brazil
[5] Univ Fed Parana, Fenn Mass Spectrometry Lab, Jandaia Do Sul, Brazil
[6] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, Piracicaba, Brazil
关键词
Eichhornia azurea; Eichhornia crassipes; endophytes; MS/MS fragmentation mechanism; FUNGI; DIVERSITY; KUNTH;
D O I
10.1080/14786419.2017.1380011
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In the present study, biological activity and chemical composition of two crude extracts of endophytic fungal strains of Bipolaris genera isolated from two species of aquatic macrophytes: Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) were investigated. The nuclear magnetic resonance and mass spectrometry data provided the identification of three main compounds: curvulin (1), spirostaphylotrichin R (2) and U (3). The fragmentation mechanism of the precursor ions towards collision induced dissociation (CID) tandem mass spectrometry experiment (MS/MS) is also proposed. Furthermore, biological screening of the crude extracts displayed antileishmanial activity with IC 50 values ranging from 70-84.2 mu g. mL(-1). [GRAPHICS] .
引用
收藏
页码:2783 / 2790
页数:8
相关论文
共 23 条
[1]   SPIROSTAPHYLOTRICHIN-U AND SPIROSTAPHYLOTRICHIN-V FROM CURVULARIA-PALLESCENS [J].
ABRAHAM, WR ;
HANSSEN, HP ;
ARFMANN, HA .
PHYTOCHEMISTRY, 1995, 38 (04) :843-845
[2]  
Ahsan M.J., 2016, Beni-Suef University Journal of Basicand Applied Sciences, V5, P119, DOI [10.1016/j.bjbas.2016.05.002, DOI 10.1016/J.BJBAS.2016.05.002]
[3]   Molecular characterization of the endophytic fungal community associated with Eichhornia azurea (Kunth) and Eichhornia crassipes (Mart.) (Pontederiaceae) native to the Upper Parana River floodplain, Brazil [J].
Almeida, T. T. ;
Orlandelli, R. C. ;
Azevedo, J. L. ;
Pamphile, J. A. .
GENETICS AND MOLECULAR RESEARCH, 2015, 14 (02) :4920-4931
[4]   A dimeric chromanone and a phthalide: Metabolites from the seagrass-derived fungus Bipolaris sp PSU-ES64 [J].
Arunpanichlert, Jiraporn ;
Rukachaisirikul, Vatcharin ;
Tadpetch, Kwanruthai ;
Phongpaichit, Souwalak ;
Hutadilok-Towatana, Nongporn ;
Supaphon, Orathai ;
Sakayaroj, Jariya .
PHYTOCHEMISTRY LETTERS, 2012, 5 (03) :604-608
[5]  
Azevedo Joao Lucio, 2000, EJB Electronic Journal of Biotechnology, V3, P1, DOI 10.2225/vol3-issue1-fulltext-4
[6]  
Castellani A, 1939, J TROP MED HYG, V42, P65
[7]   Indole-3-acetic acid (IAA) production trait, a useful screening to select endophytic and rhizosphere competent bacteria for rice growth promoting agents [J].
Etesami, Hassan ;
Alikhani, Hossein Ali ;
Hosseini, Hossein Mirseyed .
METHODSX, 2015, 2 :72-78
[8]   Molecular phylogeny, diversity, symbiosis and discover of bioactive compounds of endophytic fungi associated with the medicinal Amazonian plant Carapa guianensis Aublet (Meliaceae) [J].
Ferreira, Mariana Costa ;
Almeida Vieira, Mariana de Lourdes ;
Zani, Carlos Leomar ;
de Almeida Alves, Tania Maria ;
Sales Junior, Policarpo Ademar ;
Murta, Silvane M. F. ;
Romanha, Alvaro Jose ;
Vega Gonzales Gil, Laura Helena ;
de Oliveira Carvalho, Amanda Gomes ;
Zilli, Jerri Edson ;
Salgado Vital, Marcos Jose ;
Rosa, Carlos Augusto ;
Rosa, Luiz Henrique .
BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2015, 59 :36-44
[9]   The use of secondary metabolite profiling in chemotaxonomy of filamentous fungi [J].
Frisvad, Jens C. ;
Andersen, Birgitte ;
Thrane, Ulf .
MYCOLOGICAL RESEARCH, 2008, 112 :231-240
[10]  
García A, 2012, BIOL RES, V45, P139, DOI 10.4067/S0716-97602012000200006