Circular RNA hsa_circ_0131242 Promotes Triple-Negative Breast Cancer Progression by Sponging hsa-miR-2682

被引:24
作者
Li, Yueting [1 ]
Shi, Pengxu [2 ]
Zheng, Tianzhi [1 ]
Ying, Ziwei [1 ]
Jiang, Daqing [1 ]
机构
[1] China Med Univ, Canc Hosp, Dept Breast Surg, Shenyang 110042, Liaoning, Peoples R China
[2] Peoples Hosp Liaoning Prov, Dept Bone Surg, Shenyang 110016, Liaoning, Peoples R China
来源
ONCOTARGETS AND THERAPY | 2020年 / 13卷
关键词
breast cancer; hsa_circ_0131242; proliferation; sponge; biomarker; EXPRESSION;
D O I
10.2147/OTT.S246957
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Objective: CircRNAs are emerging as vital regulators in a variety of cancers. However, the expression pattern and potential mechanism of circRNAs in triple-negative breast cancer remain unclear. In this study, we aim to systematically investigate circRNAs alteration in triple-negative breast cancer tissues. Methods: Microarray and bioinformatics analyses were used to identify circRNAs expression in cancer tissues. qRT-PCR was conducted to measure the expression of RNAs. Cell Counting Kit-8, wound-healing and transwell assays were conducted to investigate the function of circRNAs. Dual-luciferase reporter assay was performed to validate target binding. Results: Hsa_circ_0131242 was highly expressed in both cancer tissues and cell lines compared to control. Subsequently, statistical analyses revealed that high expression of hsa_circ_0131242 was positively correlated with advanced tumor stages and poorer clinical features in cancer patients. Hsa_circ_0131242 knockdown could suppress the progression of breast cancer cells. Bioinformatics prediction and luciferase reporter assay showed that hsa_circ_0131242 acted as a sponge for hsa-miR-2682. Moreover, co-transfection of hsa-miR-2682 inhibitor and si-hsa_circ_0131242 rescued cell proliferation and migration in BT549 and MDA-MB-468 cell lines. Conclusion: Our study identified hsa_circ_0131242 expression in TNBC for the first time and found that hsa_circ_0131242 may promote triple-negative breast cancer progression by sponging hsa-miR-2682.
引用
收藏
页码:4791 / 4798
页数:8
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