Determination of citric acid in fermentation media by pyrolysis mass spectrometry

被引：15
作者：
Ghassempour, A [1 ]
Najafi, M
Amiri, AA
机构：
[1] Shahid Beheshti Univ, Fac Sci, Chem Res Ctr, Tehran, Iran
[2] Shahid Beheshti Univ, Fac Sci, Dept Chem, Tehran, Iran
来源：
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS | 2003年 / 70卷 / 02期
关键词：
pyrolysis mass spectrometry; citric acid; Aspergillus niger; factorial design;
D O I：
10.1016/S0165-2370(02)00135-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Rapid and sensitive determination of citric acid in fermentation media by pyrolysis mass spectrometry (Py-MS) is proposed. Owing to high specificity of this method, distinguishing the citric acid from the matrix and by-products formed in the Krebs cycle is possible. Selected ion monitoring (SIM) mode is used for quantitative measurements, in which mass to charge (m/z) values of 175 of citric acid and 138 of 3-nitroaniline as internal standard are chosen. Limit of detection (LOD) for this method has been found to be I ng ml(-1) and the linear working range was 10 ng ml(-1)-100 mg ml(-1). Relative standard deviation (R.S.D.) of the method for five replicates was 0.84%. Results of Py-MS are compared with those obtained by UV-vis spectrophotometric method. Also, factor analysis is used for evaluating the influence of pH, molasses concentration, time and shaker intensity on the production of citric acid by Aspergillus niger. (C) 2002 Elsevier Science B.V. All rights reserved.
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收藏
页码：251 / 261
页数：11
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